The aim of this study is to observe specimens of bronchoalveolar lavage fluid from patients using a reflection enhanced dark-field scattering microscopy. By observing parameters such as the size, morphology, scattering intensity, and movement speed of pathogens, combined with the clinical characteristics of patients, a rapid diagnosis of Pseudomonas aeruginosa infection can be made. At the same time, the bronchoalveolar lavage fluid samples of patients infected with Pseudomonas aeruginosa before and after treatment were compared, and the quantity and vitality of Pseudomonas aeruginosa were observed to judge the efficacy of antibiotics.
Study Type
OBSERVATIONAL
Enrollment
100
We will perform bronchoscopy and collect bronchoalveolar lavage fluid (BALF)
The First Affiliated Hospital of Nanjing Medical University
Nanjing, Jiangsu, China
The size of pathogens
Measure the size of pathogens
Time frame: From enrollment to the end of treatment at 2 weeks
scattering intensity of pathogen
Determine the shape of pathogens through scattering intensity
Time frame: From enrollment to the end of treatment at 2 weeks
movement speed of pathogens
Measure the movement speed of pathogens, such as flagellates like Pseudomonas aeruginosa, which have a fast movement speed
Time frame: From enrollment to the end of treatment at 2 weeks
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