Calcium Pyrophosphate Deposition (CPPD) Disease

N/ANot Yet RecruitingNCT07005804

Lille Catholic University137 enrolled

Overview

The goal of this clinical trial is to describe the transcriptomic and metabolomic profile of patients with chronic Calcium Pyrophosphate Deposition (CPPD) compared to those with acute CPPD. The hypotheses are as follows : * It is hypothesised that there is a transcriptomic and metabolomic signature of CPPD which explains why therapeutic responses to different anti-inflammatory treatments differ from one phenotype to another one * It is hypothesised that the acute and chronic clinical phenotypes of CPPD have different clinical, biological and imaging characteristics, as well as a differing predisposition toward crystalline deposition and inflammatory pathway activation. The management of participants with chronic forms of the disease included in this research was modelled on the usual recommended management, including a biological workup, joint puncture, ultrasound and radiographic workup. Double-energy CT scans and transcriptomic and metabolomic analyses on plasma are not routine tests.

Study Type

INTERVENTIONAL

Allocation

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

137

Conditions

Calcium Pyrophosphate Deposition Disease Rheumatic Diseases

transcriptomic and metabolomic analysisGENETIC

Venous blood samples for transcriptomic and metabolomic analysis will be taken in 3 x 4 mL ethylenediaminetetraacetic acid (EDTA) tubes and stored immediately at 4°C, before being cryopreserved at -80°C in 500 μL aliquots of plasma. In addition, for transcriptomic analysis, a sample will also be taken in a Paxgen RNA tube cryopreserved at -80°C.

Eligibility

Sex: ALLMin age: 65 Years

Medical Language ↔ Plain English

Inclusion Criteria - Acute forms of CPPD: retrospective part of the study * Cases included in the COLCHICORT cohort (NCT03128905), for whom this was the first acute episode of CPPD Inclusion Criteria - Chronical forms of CPPD : prospective part of the study * Patients affiliated to the French social security system * Age ≥ 65 years * Diagnosis of chronic CPPD (recurrent acute or persistent arthritis), meeting ACR/EULAR 2023,11 classification criteria after evaluation by a rheumatologist in the rheumatology department * Progression of CPPD rheumatism for at least 3 months and still active CPPD rheumatism, defined by a visual analogue scale (VAS) of disease activity ≥ 40 and/or presentation of at least 1 crisis over the last 3 months * Glomerular Filtration Rate (GFR) in Chronic Kidney Disease Epidemiology (CKD EPI Collaboration) ≥ 30ml/min/1.73m2 * Minimum time between the last intake of a crisis treatment and inclusion in the study, depending on their half-life, in order to not interfere with the results of the omics analyses: 2 weeks (Nonsteroidal Anti-Inflammatory Drugs Per Os (PO) or Intramuscular (IM) or Intravenous (IV); corticosteroids PO or IV; colchicine PO; anakinra Subcutaneous (SC), 1 month (methotrexate PO, tocilizumab SC), 3 months (canakinumab SC, tocilizumab IV) * Signed written consent for study participation Exclusion Criteria - Acute forms of CPPD: * Missing data concerning transcriptomic and metabolomic analyses. * Opposition Exclusion Criteria - For chronic forms of CPPD: * History of gout or presence of monosodium urate (MSU) crystals on joint fluid, * Cognitive decline (confusion and neurodegenerative diseases) * Inability to provide informed consent and disease VAS * Patient under guardianship or curatorship

Outcomes

Primary Outcomes

Transcriptomic profile

-Quantitative transcriptomic profiling (expression of the genes involved) and qualitative profiling (analysis of the sequence of variants expressed)

Time frame: 2 weeks

Metabolomic profile

\- Metabolomic profile: quantitative assessment of the metabolites involved in each phenotype, then qualitatively grouped by activated metabolic pathway