Phase I/II Clinical Trial of Universal Donor CD33 CAR Natural Killer Cells for AML

Inclusion Criteria: 1. Patients with relapsed or primary refractory CD33+ AML, including: * Patients with relapsed AML (patients in second or subsequent relapse, or any relapse after HSCT, are eligible). * Refractory AML defined as failure to achieve a complete response after 2 cycles of induction or reinduction chemotherapy, including persistent MRD positivity. * Patients with isolated CNS or extramedullary disease are eligible. Patients with CNS disease are excluded from the phase I dose escalation portion but are eligible for the phase II portion of the study. 2. 1-39.99 years of age (note: the first three subjects treated AND the first subject on each dose level must be ≥ 16 years of age) 3. Negative serum test to rule out pregnancy within 14 days prior to enrollment in females of childbearing potential o Sexually active males and females of childbearing potential must agree to use a form of contraception considered effective and medically acceptable by the Investigator for 6 months after the last dose of chemotherapy and/or NK cell infusion 4. Organ function requirements: * Renal function: Creatinine ≤ 2 times the institutional upper limit of normal for age OR creatinine clearance \> 60 ml/min/1.73m2 (measured by 24 hour- urine specimen or radioisotope GFR) * Liver function: Total bilirubin ≤ 2 mg/dl (unless Gilbert's syndrome), AST and ALT ≤ 5 times the upper limit of normal (unless related to leukemic involvement). Upper limit of normal should be determined by the institutional defined normal laboratory range. * Cardiac function: left ventricular ejection fraction ≥ 40% or shortening fraction ≥20%. May be eligible after cardiology clearance if qualitatively normal function or repeat measures are normal. * CNS: Patients with seizure disorder may be eligible if seizures are well controlled * Pulmonary function: baseline oxygen saturation \>92% on room air at rest 5. Due to the risk of hematopoietic toxicity from CD33 targeting, enrolled subjects must have an allogeneic HCT donor identified and be eligible and willing to undergo a subsequent HSCT in the event of aplasia. 6. All patients or their legal guardian must be able to understand and willing to sign a written informed consent document. 7. All patients must consent to enroll in a separate long term follow up study for cell and gene therapy Exclusion Criteria: 1. Prior therapies: * AML directed therapies in the 14 days prior to beginning treatment on this protocol (except for hydroxyurea) Note: There is no waiting period required for patients having received intrathecal cytarabine, methotrexate and/or hydrocortisone * Gemtuzumab or other CD33-targeted antibody within 42 days of enrollment * CNS radiation within 28 days of enrollment * DLI or adoptive cell therapy within 30 days of enrollment * Allogeneic SCT within 90 days of enrollment * Patients with CNS disease are excluded from the phase I portion of the study but are eligible for the phase II expansion phase. * Patients on immunosuppressive therapy * Patients must be off of systemic immunosuppressive therapy for at least 14 days prior to enrollment with no evidence of recurrent GVHD * Patients on hydrocortisone for treatment of adrenal insufficiency are permitted on study * Patients on corticosteroids ≤ 0.5mg/kg/day (prednisone equivalent) for any other indication are permitted on study 2. Uncontrolled, symptomatic, intercurrent illness or social situations that would limit compliance with study requirements or in the opinion of the site PI would pose an unacceptable risk to the subject 3. Patients who are breastfeeding 4. Patients with prior solid organ transplantation 5. Performance status: Karnofsky or Lansky Performance Scale (PS) \< 50 6. Uncontrolled infection, defined as an infection which has not resolved or does not show evidence of significant resolution after initiating appropriate therapy o Asymptomatic viremia such as CMV, HPV, BK virus, HCV, etc. is NOT considered as an exclusion criterion 7. Uncontrolled arrhythmias or uncontrolled symptomatic cardiac disease 8. Active acute or chronic GVHD of any grade at the time of enrollment. "Active GVHD" is defined as a patient who requires immunosuppressive therapy for control of their GVHD symptoms.