The goal of this intervention study is to evaluate the beneficial effect of the probiotic bacterium Bacillus coagulans in healthy volunteers. The main question it aims to answer is if the probiotic strains are modifying the microbiota composition in a beneficial way, evaluated in faecal- and saliva samples. Primary hypothesis: The probiotic bacteria will modify the microbiota composition in faecal- and saliva samples. Participants will consume the freeze-dried probiotic bacteria for 14 days. Before and after consumption, the participants will collect samples.
Samples will be frozen immediately after collection and will be kept frozen until analysis at the Department of Process and Life Science Engineering, Faculty of Engineering, Lund University. For diversity measures, species identification and relative abundance of bacterial taxa, samples will be analysed by Illuminas MiSeq with the MiSeq reagent kit v3. Data will be analysed with bioinformatic pipeline QIIME2TM.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
NONE
Enrollment
60
Consumption of probiotic capsules containing the probiotic bacteria Bacillus coagulans strain 5.
Consumption of probiotic capsules containing the probiotic bacteria Bacillus coagulans strain 6.
Department of Process and Life Science Engineering, Lund University
Lund, Sweden
RECRUITINGThe gut microbiota composition
Diversity index will be calculated based on the results from next generation sequencing
Time frame: At enrollment and after 2 weeks of consumption.
Relative abundance
Quantification of bacterial taxa will be calculated based on the results from next generation sequencing
Time frame: At enrollment and after 2 weeks of consumption.
This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.