Pathological cardiac hypertrophy is characterized by abnormal cardiomyocyte metabolism, reduced myocardial contractility, and dysregulated synthesis of myocardial contractile proteins. This pathological process leads to progressive impairment of cardiac function and ultimately progresses to heart failure. Previous studies have demonstrated that PRMT5 exerts a significant inhibitory effect on heart failure, yet its clinical significance in the context of heart failure remains undefined. In this study, we hypothesized that serum PRMT5 may serve as a biomarker to predict cardiac structural parameters and functional indices. Therefore, we aim to analyse the correlation between serum PRMT5 levels and the following parameters-LVPWs, LVPWd, LVIDs, LVIDd, IVSTs, IVSTd, EF, FS, LVMi and RWT on the first day when participants are enrolled in this study.
The aim of this study is to collect blood samples from both healthy controls and heart failure patients and to clarify the correlation between serum PRMT5 levels on the first day of enrollment and cardiac ultrasound indicators. Serum PRMT5 levels are measured by means of enzyme-linked immunosorbent assay (ELISA). Left ventricular posterior wall thickness at end-systole (LVPWs), Left ventricular posterior wall thickness at end-diastole (LVPWd), left ventricular internal diameter at end-systole (LVIDs), left ventricular internal diameter at end-diastole (LVIDd), interventricular septum at end-systole (IVSTs) and interventricular septal septum at end-diastole (IVSTd) are measured on the first day of enrollment via echocardiography. Ejection fraction (EF), fractional shortening (FS), left ventricular mass index (LVMi), and relative wall thickness (RWT) are subsequently calculated based on LVIDd, LVPWd, and IVSTd values. Finally, the correlations between serum PRMT5 levels and the following indicators are analyzed: LVPWs, LVPWd, LVIDs, LVIDd, IVSTs, IVSTd, EF, FS, LVMi and RWT.
Study Type
OBSERVATIONAL
Enrollment
50
the University of Hongkong-Shenzhen Hospital
Shenzhen, Guangdong, China
Serum PRMT5 level at baseline
Serum PRMT5 level is analysed at the day of informed consent agreement and blood sampling
Time frame: Baseline (day of informed consent agreement and blood sampling)
LVIDs level at baseline
Left ventricular internal diameter at systolic state is measured at the day of informed consent agreement and cardiac ultrasound examination
Time frame: Baseline
LVIDd level at baseline
Left ventricular internal diameter at diastolic state is measured at the day of informed consent agreement and cardiac ultrasound examination
Time frame: Baseline
LVPWs level at baseline
left ventricular posterior wall at systolic state is measured at the day of informed consent agreement and cardiac ultrasound examination
Time frame: Baseline
LVPWd level at baseline
left ventricular posterior wall at diastole state is measured at the day of informed consent agreement and cardiac ultrasound examination
Time frame: Baseline
IVSTs at baseline
Interventricular septum at systolic state is measured at the day of informed consent agreement and cardiac ultrasound examination
Time frame: Baseline
IVSTd at baseline
Interventricular septum at diastolic state is measured at the day of informed consent agreement and cardiac ultrasound examination
Time frame: Baseline
EF at baseline
Ejection fraction is calculated according to the formula below: 1. EF=(LVEDV-LVESV)/LVEDV\*100%; 2. LVEDV=(7.0\*LVIDd\^3)/(2.4+LVIDd) 3. LVESV=(7.0\*LVIDs\^3)/(2.4+LVIDs)
Time frame: Baseline
FS at baseline
Fractional shortening is calculated based on the formula below: FS=(LVIDd-LVIDs)/LVIDd\*100%
Time frame: Baseline
LVMi at baseline
Left ventricular mass index is calculated according to the formula below: 1. LVMi(g/m\^2)=LVM/BSA; 2. LVM(g)=LVM=0.8×1.04×\[(LVIDd+IVSd+LVPWd)\^3-LVIDd\^3\]+0.6; 3. BSA(m\^2)=0.007184×W\^0.425×H\^0.725 (W: Weight, kg; H: Height, cm)
Time frame: Baseline
RWT at baseline
Relative wall thickness is calculated according to the formula below: RWT=2\*(IVSd+LVPWd)/LVIDd
Time frame: Baseline
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