The Characteristics and Treatment Mechanism of Vascular Grafts in Elderly Patients With CAOD Through Single-cell Transcriptome Analysis

Yonsei University100 enrolled

Overview

This study aims to establish a cardiovascular biobank using vascular grafts obtained from participants with coronary artery occlusive disease (CAOD). Through genomic and transcriptomic analyses, the study seeks to characterize the biological features of the vascular grafts and identify potential therapeutic targets and mechanisms related to vascular remodeling and stiffness.

This observational study will utilize discarded vascular graft tissues obtained during coronary artery bypass grafting (CABG) procedures in participants with coronary artery occlusive disease (CAOD). Our surgical team performs over 200 CABG operations annually, allowing for the consistent and ethical acquisition of excess graft tissue without requiring any additional procedures or interventions for participants. Collected tissues will be processed as follows: A portion of each graft will be preserved in RNAlater solution for molecular analyses, including total RNA sequencing, quantitative PCR, and western blotting. Another portion will be fixed in formalin and embedded for histological examination, including immunohistochemistry (IHC), to validate mechanisms of vascular remodeling and stiffness at the protein level. This approach ensures that all biological samples are collected with minimal risk and no additional burden to participants, while enabling comprehensive multi-omic analysis to investigate the pathophysiological characteristics of vascular grafts in elderly CAOD patients.

Study Type

OBSERVATIONAL

Enrollment

100

Conditions

Coronary Artery Occlusive Disease (CAOD)

Eligibility

Sex: ALLMin age: 19 YearsMax age: 100 Years

Medical Language ↔ Plain English

Inclusion Criteria: * Adults aged 19 years or older, Patients scheduled to undergo coronary artery bypass grafting (CABG), Patients who voluntarily provide written informed consent for study participation, Patients who are able and willing to comply with study procedures during the study period Exclusion Criteria: * Pediatric or adolescent patients under 18 years of age, Patients requiring concomitant surgical correction for valvular heart disease, Patients undergoing reoperative (redo) cardiac surgery, Patients who decline to provide informed consent prior to surgery

Locations (1)

Division of Cardiovascular Surgery, Severance Cardiovascular Hospital , Yonsei University College of Medicine

Seoul, South Korea

RECRUITING

Outcomes

Primary Outcomes

Identification of gene expression profiles associated with vascular stiffness in graft tissues

To identify differentially expressed genes and signaling pathways related to vascular stiffness using total RNA sequencing of vascular graft tissues obtained during coronary artery bypass grafting (CABG).

Time frame: At the time of surgery (single timepoint sample collection)

Secondary Outcomes

Validation of stiffness-related gene targets and correlation with clinical and histological parameters

To validate selected stiffness-associated gene targets identified through RNA sequencing using qPCR and western blotting; to assess corresponding protein expression through immunohistochemistry; and to correlate transcriptomic findings with participants' clinical characteristics such as age, hypertension, and diabetes.

Time frame: Within 2 year after tissue collection

Data from ClinicalTrials.gov

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