Abstract: Introduction: The deepithelialized free gingival graft (DGG) technique provides high-quality connective tissue grafts (CTGs) with predictable outcomes for recession cov-erage. This study evaluates a novel method of free gingival graft (FGG) deepithelialization using an Er,Cr:YSGG laser (LDEE) for treating multiple gingival recessions. Methods: A split-mouth study was conducted on 46 (n=46) recessions in 9 patients (23 per test and control group). Sites were randomized. Full-thickness palatal grafts were harvested with a scalpel. In the test group (LDEE), deepithelialization was performed extraorally using an Er,Cr:YSGG laser (2780 nm; 2.5 W, 83.3 mJ, 30 Hz, 600 µm tip). In the control group (DEE), a 15c scalpel was used. All CTGs were applied using the modified coronally advanced tunnel (TUN) technique. Clinical parameters-recession depth (RD), keratinized tissue width (KT), gingival thickness (GT), pocket depth (PD), clinical attachment loss (CAL), pink esthetic score (PES), approximal plaque index (API), mean root coverage (MRC), and complete root coverage (CRC)-were assessed at baseline (T0), 3 months (T1), and 6 months (T2). Results: Both LDEE and DEE groups showed significant improvements in RD, KT, GT, PD, and CAL over time (p \< 0.001). At T1 and T2, KT was significantly higher in the LDEE group (T1: 3.73±0.72 mm; T2: 3.98±0.76 mm) compared to the DEE group (T1: 3.21±0.61 mm; T2: 3.44±0.74 mm; p \< 0.05). Other parameters (RD, GT, PD, CAL) showed no statistically significant intergroup differences at any time point (p \> 0.05). After 6 months, MRC was 95% and CRC 82.6% for LDEE, compared to 94.8% and 82.6% for DEE (p \> 0.05). PES scores were similar between groups at all time points (p \> 0.05). Conclusion: Both laser- and scalpel-deepithelialized grafts effectively treated gingival recessions. LDEE combined with TUN resulted in significantly greater KT width compared to DEE + TUN.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
SINGLE
Enrollment
9
Extraoral deepithelialization of harvested free gingival graft using Er,Cr:YSGG laser (2780 nm, 2.5 W, 83.3 mJ, 30 Hz, 600 μm tip) before placement in gingival recession treatment.
Extraoral deepithelialization of harvested free gingival graft using a 15c surgical scalpel blade before placement in gingival recession treatment.
Dentoplex
Bielsko-Biala, Poland
Change in Recession Depth
Measurement of gingival recession depth in millimeters to evaluate soft tissue response to treatment. Unit of Measure: mm
Time frame: Baseline, 3 months, and 6 months postoperatively
Change in Keratinized Tissue Width
Measurement of the width of keratinized gingiva in millimeters from the mucogingival junction to the free gingival margin. Unit of Measure: mm
Time frame: Baseline, 3 months, and 6 months postoperatively
Change in Gingival Thickness
Assessment of gingival tissue thickness using a standardized probe. Unit of Measure: mm
Time frame: Baseline, 3 months, and 6 months postoperatively
Change in Pocket Depth
Probing depth measured from the gingival margin to the base of the periodontal pocket. Unit of Measure: mm
Time frame: Baseline, 3 months, and 6 months postoperatively
Change in Clinical Attachment Level
Measurement of the distance from the cementoenamel junction to the base of the periodontal pocket. Unit of Measure: mm
Time frame: Baseline, 3 months, and 6 months postoperatively
Change in Pink Esthetic Score
Percentage of interdental spaces with visible plaque, calculated to assess oral hygiene status. A lower percentage indicates better plaque control. Unit of Measure: percentage (%)
Time frame: Baseline, 3 months, and 6 months postoperatively
Mean Root Coverage
Average percentage of root surface covered by soft tissue following treatment, calculated across all treated sites. Unit of Measure: percentage (%)
Time frame: 6 months postoperatively
Complete Root Coverage
Proportion of treated sites that achieved 100% root coverage postoperatively. Unit of Measure: percentage (%)
Time frame: 6 months postoperatively
Histopathological Evaluation of Laser-Treated Connective Tissue Grafts
Microscopic assessment of grafts treated with Er,Cr:YSGG laser to evaluate the quality of deepithelialization and potential thermal damage. Parameters included the presence of a clear boundary between epithelium and connective tissue, absence of carbonization or necrosis, and preservation of tissue architecture.
Time frame: Immediately postoperatively
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