Clinical Study of a Novel Humanized CD70-Targeted CAR-T Cell Incorporating TLR2 for Advanced Renal Cell Carcinoma Therapy

Inclusion Criteria: * 1\. Voluntary participation with written informed consent provided by the patient or legally authorized representative; * 2.Age 18-75 years (inclusive) at the time of consent, regardless of sex; * 3.Advanced-stage renal cell carcinoma (RCC) with no curative treatment options, who have received ≥1 prior line of therapy and meet one or more of the following: 1. Recurrence after first-line or later-line treatment(s). 2. Progression or persistent progression following prior therapy; * 4.Histopathologically confirmed advanced RCC per WHO 2016 classification, with at least one measurable lesion evaluable by CT or MRI; * 5.CD70 positivity in tumor tissue confirmed by immunohistochemistry (IHC); * 6.Adequate organ function: Hepatic: ALT/AST \<3× ULN and total bilirubin ≤34.2 μmol/L. Renal: Creatinine clearance (Cockcroft-Gault) ≥60 mL/min. Pulmonary: Oxygen saturation ≥95% with no active pulmonary infection. Cardiac: LVEF ≥50%, no significant pericardial effusion, and no clinically relevant ECG abnormalities; * 7.Contraception: Women of childbearing potential must have a negative pregnancy test (urine/serum) at screening and agree to use effective contraception for ≥1 year post-infusion. Men with partners of childbearing potential must use barrier contraception for ≥1 year post-infusion; * 8.Performance status: ECOG score 0-3; * 9.Life expectancy \>3 months; * 10.Willingness to comply with leukapheresis, medical assessments, and follow-up visits. Exclusion Criteria: * 1.Pregnant or lactating women; * 2.Uncontrolled fungal, bacterial, Treponema pallidum, viral, or other infections; * 3.Active hepatitis: HBV DNA \>500 IU/mL. Positive HCV RNA (confirmed by repeat testing); * 4.HIV infection, known acquired immunodeficiency syndrome (AIDS), or syphilis infection; * 5.Prior gene therapy of any form; * 6.History of severe allergic reactions to biologics (including antibiotics), antibodies, cytokines, or other macromolecular agents; * 7.Clinically significant CNS disorders: epilepsy, paresis, aphasia, stroke, severe traumatic brain injury, dementia, Parkinson's disease, cerebellar disorders, organic brain syndrome; * 8.Uncontrolled psychiatric illness; * 9.Substance abuse/addiction; * 10.Prohibited medications/treatments: Corticosteroids: ≥2 mg/kg prednisone (or equivalent \>20 mg/day) within 2 weeks before leukapheresis. Chemo/radiotherapy: Anti-tumor radiotherapy or salvage chemotherapy within 3 weeks before leukapheresis. Immunosuppressants: Use within 4 weeks before leukapheresis. Other trials/major surgery: Participation in another clinical trial or major non-diagnostic surgery within 4 weeks before leukapheresis. Specific agents: Alemtuzumab within 6 months, or clofarabine/cladribine within 3 months before leukapheresis.