Sepsis and Thiol-Disulfide Balance in Emergency Department

Sakarya University100 enrolled

Overview

The aim of this study was to determine the effectiveness of thiol-disulfide homeostasis in predicting diagnosis and mortality in sepsis patients, and to compare it with other inflammatory markers.

Sepsis is a serious illness with high mortality rates triggered by infection-associated pathogenesis involving oxidative stress dysfunction. This study aims to elucidate the diagnostic efficacy of changes in thiol-disulfide homeostasis, its predictive role in mortality, relationship with other inflammatory parameters in sepsis patients in emergency department. In prospective, randomized controlled study, 50 patients diagnosed with sepsis according to the Sepsis-3 guidelines who presented emergency department were compared with a control group of 50 healthy volunteers. Thiol-disulfide homeostasis parameters, including total thiol, native thiol, disulfide levels, reduced thiol, oxidized thiol, and thiol oxidation-reduction ratios, were examined. Additionally, inflammatory blood parameters (C-reactive protein, procalcitonin, lactate) and mortality durations were compared with thiol-disulfide homeostasis. The objective of this study was to examine the effectiveness of thiol-disulfide homeostasis in forecasting diagnosis and mortality in sepsis patients, and to compare it against other inflammatory markers.

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

DIAGNOSTIC

Masking

NONE

Enrollment

100

Conditions

Sepsis Oxidative Stress

Interventions

Thiol- Disulfide Blood Sample MeasurementDIAGNOSTIC_TEST

Measurement of thiol-disulfide homeostasis parameters and inflammatory markers from serum samples.

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Patients aged 18 and above who met the diagnostic criteria were included in the study Exclusion Criteria: * Who did not meet the criteria for sepsis diagnosis according to the quick Sequential Organ Failure Assessment score * Individuals with malignancy, acute coronary syndrome, acute cerebrovascular event, acute/chronic kidney/liver failure, rheumatologic diseases, febrile neutropenia, immunosuppressive disorders, corticosteroid use, and those * Individuals with a history of antibiotic use within the last week

Locations (1)

Sakarya University

Sakarya, Adapazarı, Turkey (Türkiye)

Outcomes

Primary Outcomes

Diagnostic value of serum thiol-disulfide homeostasis parameters in sepsis

Measurement of serum total thiol, native thiol, disulfide, reduced thiol, and oxidized thiol levels in patients with sepsis and healthy controls at the time of admission, and comparison between groups to evaluate diagnostic performance.

Time frame: 2 monhts

Secondary Outcomes

General Characteristics of All Participants

Demographic data (age, gender, body mass index) were recorded.

Time frame: 1 week

Correlation of inflammatory and biochemical markers with thiol-disulfide homeostasis in sepsis

Initial blood samples collected from sepsis patients were analyzed to measure total thiol, native thiol, disulfide, reduced thiol, and oxidized thiol levels. These thiol-disulfide homeostasis parameters were then compared and correlated with standard laboratory inflammatory markers, including C-reactive protein (CRP), procalcitonin, and lactate, to evaluate their relationships.

Time frame: 10 days

Data from ClinicalTrials.gov

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