This study aims to evaluate oxidative DNA damage among hookah smokers compared to non-smokers by measuring biomarkers of oxidative stress in blood samples. The objective is to determine the impact of hookah smoking on DNA integrity and overall oxidative status in adults.
Hookah smoking has become increasingly popular in many regions, yet its potential impact on oxidative stress and DNA integrity is not fully understood. This study aims to investigate the level of oxidative DNA damage among hookah smokers compared to non-smokers by analyzing biomarkers in blood samples. A case-control observational design will be used, involving two groups of adult participants: regular hookah smokers and age-matched non-smokers. Blood samples will be collected from all participants and analyzed for markers of oxidative stress, including 8-hydroxy-2'-deoxyguanosine (8-OHdG) and malondialdehyde (MDA), using validated biochemical methods. The findings are expected to provide insights into the biological consequences of hookah smoking and support the development of public health interventions.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
96
University of Duhok
Duhok, semel, Iraq
Oxidative DNA damage
Measurement of plasma MDA concentration using the thiobarbituric acid reactive substances (TBARS) method to assess oxidative stress in participants before and after 1-month Vitamin C supplementation and measuring oxidative stress marker and TAOC
Time frame: Baseline and after 1 month
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