Animal and Microbial-based Dietary Protein Efficiency in Adults

Daniel Moore13 enrolled

Overview

Given the relatively high carbon footprint and sustainability of animal-based proteins, there is a growing interest in determining the nutritional quality non-animal-based protein sources. The overall objective of this investigation is to examine the impact of different animal and microbial-based protein sources to support whole body protein synthesis in adults. To do this, investigators will employ a 'breath test' method developed in our laboratory as well as urine sampling. The results of this study will allow us to better understand the impact of dietary protein quality for maintaining health and body protein mass in adults.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

SINGLE

Enrollment

Conditions

Protein Metabolism

Interventions

Eligibility

Sex: ALLMin age: 18 YearsMax age: 45 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Healthy, male and female, recreationally-active participants. * Healthy will be defined as screened by the PAR-Q+ (Appendix B) and The Physical Activity Readiness Questionnaire (Appendix C). * Participants will be aged 18-45 years old. * Participants are willing to abide by the compliance rules of this study. * Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants). * Use of monophasic combined oral contraceptives (COC) containing 21 active pills (e.g., Yaz, Marvelon, Cyclen, etc.; the brand of COC will be recorded) (female participants). Exclusion Criteria: * Inability to adhere to any of the compliance rules judged by principal investigator (e.g. dairy protein or yeast allergy). * Self-reported regular tobacco use. * Self-reported illicit drug use (e.g., growth hormone, testosterone, etc.). * Individuals who have participated in studies within the past year involving any of the stable isotopes in the study. * Use of multiphasic COCs due to the difficulty of controlling for hormonal fluctuations in such formulations (female participants). * Use of COCs containing \>21 active pills per cycle due to potential differences in their effect on metabolism (female participants). * Use of other forms of hormonal contraception (e.g., progestin-only pill, hormonal IUD, intravaginal ring) (female participants). * Not currently or previously (in the past 6 months) on a vegan diet

Outcomes

Primary Outcomes

1. Phenylalanine excretion (umol/kg/h)

Whole body phenylalanine excretion determined from the product of breath 13CO2 enrichment and carbon dioxide production rate (VCO2).

Time frame: 8 hours

Phenylalanine oxidation (umol/kg/h)

Whole-body phenylalanine oxidation determined from the correction of phenylalanine excretion by the phenylalanine precursor enrichment determined from urine enrichment over the 8 hour measurement period.