Modern lifestyles often involve long periods of sitting, which can increase the risk of heart problems, obesity, and other health issues. Diet also plays a key role in heart health. Coffee and hibiscus tea are two common beverages, but their effects during prolonged sitting are not well understood. This study will examine how drinking coffee versus hibiscus tea affects blood pressure, heart rate, and heart rate variability in men and women during extended periods of sitting. Participants (30 in total: 15 women and 15 men) will take part in a randomized crossover study, meaning each person will try both beverages at different times. Data will be collected using questionnaires, body measurements, and devices to measure heart rate, blood pressure, and heart rate variability. The goal is to better understand how these drinks influence heart health during sedentary behavior.
Modern lifestyles, shaped by technological advancements and urbanization, have led to a dramatic increase in sedentary behavior, particularly prolonged sitting. Physical inactivity is a major risk factor for cardiovascular diseases (CVDs), obesity, and premature mortality, with women generally being less active than men. Prolonged sitting affects key indicators of cardiovascular health, including blood pressure, heart rate, and heart rate variability (HRV), which reflect autonomic nervous system regulation and overall heart function. Despite the growing concern, research on how sedentary behavior specifically affects women, particularly in Saudi Arabia, remains limited. Dietary habits also play a critical role in cardiovascular health. Coffee, one of the most widely consumed beverages worldwide, contains caffeine and polyphenols that can both stimulate and protect the cardiovascular system. Moderate coffee consumption may provide benefits such as reduced risk of chronic diseases and lower resting heart rate, while excessive intake can increase blood pressure, particularly in hypertensive individuals. Hibiscus tea, rich in flavonoids and antioxidants like anthocyanins, has been traditionally used as an antihypertensive agent. It has potential therapeutic effects, including lowering blood pressure, improving metabolic health, and protecting against cardiovascular inflammation. However, the acute effects of these beverages during prolonged sitting are not well understood. This study aims to investigate the effects of coffee versus hibiscus tea on blood pressure, heart rate, and HRV in healthy women during extended periods of sitting. Using a randomized crossover design, participants will consume each beverage at separate times while their cardiovascular responses are monitored. Data will be collected through questionnaires, body composition measurements, and non-invasive devices to track blood pressure and heart rate variability. By comparing the acute effects of coffee and hibiscus tea during sedentary behavior, this study seeks to provide new insights into how common dietary choices may influence cardiovascular function and help guide recommendations for healthier lifestyles in populations at risk of prolonged sitting.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
NONE
Enrollment
30
Participants will consume 3 cups of Arabica coffee (Guatemalan origin), providing a total of 400 mg of caffeine. Each cup is prepared with 6% coffee grounds per 100 mL of water, brewed at 90°C for 6 minutes using an electric drip coffee maker, yielding 100.8 mg caffeine per 100 mL. Immediately following coffee consumption, participants will engage in uninterrupted sitting for 3 hours. Blood pressure, heart rate, and other study questionnaires will be measured at baseline (before coffee), 1 hour after coffee intake, and hourly during the 3-hour sitting period.
Participants will consume 3 servings of hibiscus leaves tea per day, each serving containing 1.25 g of dried hibiscus leaves, totaling 3.75 g per session. The tea will be prepared by steeping the leaves in 240 mL of boiling water (100°C) for 10 minutes. Immediately after consuming the hibiscus tea, participants will engage in uninterrupted sitting for 3 hours. Blood pressure, heart rate, and other study questionnaires will be measured at baseline (before tea), 1 hour after tea intake, and hourly during the 3-hour sitting.
Princess Nourah University Lifestyle Center
Riyadh, Saudi Arabia
Systolic and diastolic blood pressure
Systolic and diastolic blood pressure will be assessed using an oscillometric blood pressure monitor (Omron HEM 7124 CP, Omron Healthcare, Illinois, USA). Measurements will be conducted in accordance with the guidelines of the American Heart Association (AHA) and the American College of Cardiology (ACC).
Time frame: Blood pressure will be recorded at five standardized time points: prior to consumption of coffee or hibiscus tea, one hour post-consumption, and following one, two, and three hours of prolonged sitting.
Heart Rate
Heart rate will be measured using the Polar V800 system, which consists of a wristwatch and a Bluetooth heart rate sensor. The sensor will be positioned on the participant's chest, just below the pectoral muscles. Participants will remain seated quietly for 10 minutes while continuous heart rate data are recorded according to the manufacturer's instructions. The clearest five-minute segment of the 10-minute recording will be analyzed using Kubios HRV Premium software to assess heart rate (beat/minute)
Time frame: Heart rate measurements will be performed at multiple time points during each visit: prior to beverage consumption, one hour post-consumption, and after one, two, and three hours of prolonged sitting.
NN Intervals
NN Intervals will be measured using the Polar V800 system, which consists of a wristwatch and a Bluetooth heart rate sensor. The sensor will be positioned on the participant's chest, just below the pectoral muscles. Participants will remain seated quietly for 10 minutes while continuous heart rate data are recorded according to the manufacturer's instructions. The clearest five-minute segment of the 10-minute recording will be analyzed using Kubios HRV Premium software to assess NN Intervals (milliseconds)
Time frame: NN Intervals measurements will be performed at multiple time points during each visit: prior to beverage consumption, one hour post-consumption, and after one, two, and three hours of prolonged sitting.
RMSSD
RMSSD will be measured using the Polar V800 system, which consists of a wristwatch and a Bluetooth heart rate sensor. The sensor will be positioned on the participant's chest, just below the pectoral muscles. Participants will remain seated quietly for 10 minutes while continuous heart rate data are recorded according to the manufacturer's instructions. The clearest five-minute segment of the 10-minute recording will be analyzed using Kubios HRV Premium software to assess RMSSD (milliseconds squared)
Time frame: RMSSD measurements will be performed at multiple time points during each visit: prior to beverage consumption, one hour post-consumption, and after one, two, and three hours of prolonged sitting.
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