Hydration Status and Ocular Parameters Study

N/ANot Yet RecruitingNCT07177131

Ante Kreso60 enrolled

Overview

This study will examine how hydration status (drinking a large amount of water or avoiding water for a short period of time) affects the eye. Healthy adult volunteers will participate in two sessions: one after mild dehydration (no water for 12 hours) and one after drinking 2 liters of water within 4 hours. At each session, detailed eye measurements will be taken, including scans of the retina and optic nerve using optical coherence tomography (OCT), corneal imaging with Pentacam, and eye length measurements with an optical biometer. The results from the two conditions will be compared within the same participants. The main goal is to determine whether hydration influences the thickness of the retina (macula). Secondary goals include assessing changes in the optic nerve, cornea, and other biometric eye parameters. The study involves only non-invasive tests and minimal risk. Findings may help improve understanding of how hydration affects the eye and may be important for clinical practice, such as planning cataract or refractive surgery.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

SINGLE

Enrollment

Conditions

Hydration Status Macular Thickness Ocular Biometry

Interventions

Relative Dehydration ProtocolBEHAVIORAL

Participants will abstain from water intake for 12 hours prior to the study visit. Food is allowed, but fluid intake is limited to a maximum of 250 ml until 20:00 h on the previous evening. Eye measurements will be performed in the morning (8-10 h).

Hyperhydration ProtocolBEHAVIORAL

Participants will consume 2 liters of water within 4 hours prior to the study visit. Eye measurements will be performed in the morning (8-10 h).

Eligibility

Sex: ALLMin age: 18 YearsMax age: 65 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Healthy adult volunteers aged 18 to 65 years * Normal ophthalmological findings * Best corrected visual acuity (BCVA) ≥ 1.0 (decimal) * Willingness to participate and provide written informed consent Exclusion Criteria: * Known ocular diseases (e.g., glaucoma, keratoconus, retinal pathology) * History of ocular surgery * Systemic diseases affecting hydration status (e.g., diabetes mellitus, kidney disease) * Any condition that, in the investigator's opinion, could interfere with study participation or data quality * Refusal to participate

Outcomes

Primary Outcomes

Change in macular thickness between dehydration and hyperhydration states

Macular thickness (central subfield, parafoveal, and perifoveal regions) will be measured using Zeiss Cirrus HD-OCT. Each participant will undergo measurements in both hydration states, and paired comparisons will be performed.

Time frame: Within 1 week (cross-over design with ≥7-day washout period)