Moderate-intensity physical exercise is widely recognized for its health benefits, yet time constraints limit adherence. High-intensity interval training (HIIT) has gained popularity as a more time-efficient alternative, eliciting significant cardiovascular, respiratory, metabolic, and neuromuscular responses. More recently, functional strength exercises have been integrated into high-intensity training, leading to high-intensity functional training (HIFT) and moderate-intensity functional training (MIFT). However, the acute physiological responses to these modalities remain underexplored compared to traditional HIIT (running or cycling) and strength training. This study aims to assess and compare the acute cardiovascular, metabolic, and neuromuscular responses of HIFT, MIFT, HIIT, and traditional strength training in healthy, physically active adults to inform their potential application in special populations.
Moderate-intensity physical exercise has long been a cornerstone in promoting health and well-being. However, despite its well-documented benefits, an exclusive focus on this training modality may overlook some important limitations. One such limitation is the challenge of dedicating sufficient time to physical activity to achieve health and fitness goals. Given this constraint, recent years have witnessed a significant shift in training modalities, moving away from continuous moderate-intensity exercise approaches \[e.g., 55-70% of peak heart rate (HRpeak) or 40-60% of maximal oxygen uptake (VO₂max)\] toward higher-intensity interval training protocols (e.g., \>90% HRpeak or \>85% VO₂max), thereby enhancing time efficiency and enjoyment for practitioners. In this context, the most common type of training is high-intensity interval training (HIIT). HIIT consists of performing brief, high-intensity cyclic and continuous efforts (e.g., running or cycling) separated by predefined rest periods while using a single exercise modality. These short efforts (e.g., 1 to 4 minutes) at high intensity, whether cycling or running, elicit substantial cardiovascular, respiratory, metabolic, and neuromuscular stimuli, which can persist for hours after the workout. Recently, this training approach has incorporated functional strength exercises-movements that mimic natural movement patterns and have practical applications in daily life-performed at high intensity \[e.g., \>75% of one-repetition maximum (1RM)\] and following a circuit-based exercise organization (i.e., performing one exercise after another rather than using simple sets and pauses), a modality commonly referred to as high-intensity functional training (HIFT). Reducing the intensity of prescribed functional exercises (e.g., 60% 1RM) brings the training program closer to the demands of daily activities, a modality commonly known as moderate-intensity functional training (MIFT). However, both HIFT and MIFT define their intensity theoretically, based on the relative intensity concerning the maximum dynamic concentric strength that individuals can develop in each exercise (%1RM). Meanwhile, the cardiovascular, respiratory, metabolic, and neuromuscular demands of these methodologies remain largely unexplored compared to running-based HIIT (HIIT-R), cycling-based HIIT (HIIT-C), and traditional strength training with moderate (M-FT) and high (H-FT) loads in healthy, physically active adults. Therefore, before these methods can be applied to special populations (e.g., individuals with chronic cardiovascular or respiratory diseases, cancer, or older adults), it is essential to first understand their acute response in healthy, physically active individuals.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
OTHER
Masking
SINGLE
Enrollment
27
Six sets of 2-minute runs will be performed at an intensity of 90-95% of the maximal aerobic speed (MAS) on a treadmill. The recovery between each set will be 2 minutes, during which the subject will walk at an intensity of 50-60% of MAS.
Six sets of 2-minute cycling will be performed at an intensity of 90-95% of the maximal heart rate on a stationary bike. The recovery between each set will be 2 minutes, during which the subject will cycle at an intensity of 50-60% of maximal heart rate.
Three sets of six repetitions will be performed at 80% of the one repetition-maximum (1-RM) for six exercises. The recovery between exercises will be the minimum time required to switch from one exercise to the next, and between sets, it will be two minutes. Participants will be instructed to move the load as quickly as possible during each repetition.
Three sets of six repetitions will be performed at 60% of the 1-RM for six exercises. The recovery between exercises will be the minimum time required to switch from one exercise to the next, and between sets, it will be two minutes. Participants will be instructed to move the load as quickly as possible during each repetition.
Three sets of six repetitions will be performed at 60% of the 1-RM for six exercises. The recovery after each set will be two minutes of passive rest. Participants will be instructed to move the load as quickly as possible during each repetition.
Three sets of six repetitions will be performed at 80% of the 1-RM for six exercises. The recovery after each set will be two minutes of passive rest. Participants will be instructed to move the load as quickly as possible during each repetition.
Miguel de Cervantes European University
Valladolid, Valladolid, Spain
RECRUITINGHeart rate variability
The heart rate variability (HRV) of the participants will be assessed by monitoring their heart rate, which reflects the variation in the intervals between heartbeats (R-R intervals). This measure is a useful tool in monitoring patient health, as it estimates the balance between the sympathetic and parasympathetic activity of the autonomic nervous system. To evaluate heart rate variability, a Polar H10 heart rate monitor (Polar Electro Oy, Kempele, Finland) and a mobile application, specifically Elite HRV, will be used. Heart rate variability will be monitored daily during the week leading up to the initial assessment visits. Participants will be instructed to take the measurement for the first 5 minutes upon waking and while fasting. Subsequently, heart rate variability will be assessed immediately before each training condition, as well as immediately after, at 2, 24, 48, and 72 hours after finishing each training condition.
Time frame: Week 0 (baseline), Weeks 1-4 (during intervention and 24, 48, and 72 hours post-intervention)
Post-exercise oxygen debt
The oxygen debt (EPOC) will be calculated every minute during the 12 minutes following the completion of exercise using the following formula: EPOC (L·min-¹) = oxygen consumption (VO2) post-exercise (L·min-¹) - VO2 at rest (L·min-¹). Subsequently, EPOC will be converted into energy expenditure, using the conversion of 1L of O2 = 4.64 kcal, to exclude the rapid glycolytic resynthesis of adenosine triphosphate (ATP) as part of the conversion of O2 consumption into energy expenditure (EE).
Time frame: Weeks 2-4 (after every intervention session)
Delayed onset muscle soreness
This will be recorded using a visual analog scale, with a score ranging from 0 (no pain) to 10 (worst possible pain), immediately after the exercise and at 24, 48, and 72 hours after completing the training session. To quantify the pain and standardize the measurements, participants will be asked to perform 5 rapid repetitions of sitting down and standing up from a chair, as well as 5 rapid push-up repetitions, after which they will respond to the question, "How sore are your legs/arms?".
Time frame: Weeks 2-4 (24, 48, and 72 hours post-intervention)
Satisfaction with the type of training
It will be evaluated at the end of each session using a numerical scale from 0 to 10, where the highest score indicates maximum satisfaction with the type of training.
Time frame: Weeks 2-4 (right after every intervention session and 24 hours post-intervention)
Subjective post-exercise fatigue
It will be measured using the perceived fatigue scale (ROF). This is a numerical scale that ranges from 0 (no fatigue) to 10 (total fatigue or exhaustion), with five descriptors and five diagrams to help the individual understand the scale and make an assessment. This evaluation will be conducted immediately after the exercise session and 24 hours after completion.
Time frame: Weeks 2-4 (right after every intervention session and 24 hours post-intervention)
Perceived effort
It will be monitored during the six experimental conditions using the Rate of Perceived Exertion (RPE CR-10) scale. The RPE will be recorded after each set or circuit round. Immediately after completing the last set of each condition, participants will be asked to indicate their perceived effort for the entire session. Subsequently, it will be recorded at 24, 48, and 72 hours after the completion of each condition.
Time frame: Weeks 2-4 (right after every intervention session and 24, 48, and 72 hours post-intervention)
Gas exchange analysis
The values of mean and peak gas exchange (ml·kg-1·min-1) during the intervention sessions. The gas analyzer that will be used in this study (Cortex Metalyzer 3B, Leipzig, Germany).
Time frame: Weeks 2-4 (during intervention sessions)
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