The aim of this study is to investigate different resistance training loads on BNDF and irisin levels in platelets, blood serum and plasma.
In this study, the investigators will examine how three resistance training protocols acutely affect BDNF levels (in serum, plasma, platelet count, and BDNF per platelet) and irisin levels in blood (plasma). The resistance training protocols will differ in intensity, rest intervals between sets and repetition execution. Blood samples will be collected from participants at four time points: before the start of the protocol, 5 minutes after completion of the final set, 1 hour after completion of the final set, and 3 hours after completion of the final set. In addition, blood lactate levels will be monitored at the same time points. Heart rate will also be recorded (immediately before the start of the protocol and 30 seconds after completion of the final set). A control protocol will also be implemented, in which blood will be collected at the same time points. Data will be collected across five visits to the kinesiology laboratory at the University of Ljubljana, Faculty of Sport. Each visit will last approximately 4 hours, except for the first visit where 1RM will be measured.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
24
Participant will do resistance exercise at 80% 1RM with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.
Participant will do resistance exercise at 80% 1RM with 1 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.
Participant will do accenuated eccentric resistance exercise at 80% 1RM in concetric part of movement and 110% 1RM in eccentric part of movement with 2 minutes break, 4 sets of each exercise. Included exercises are lat pull, bench press and leg press.
University of Ljubljana, Faculty of Sport
Ljubljana, Slovenia
plasma BDNF
BDNF will be determined in blood plasma with ELISA kit.
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Irisin
Irisin will be measured in blood plasma.
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
serum BDNF
BDNF will be determined in blood serum with ELISA kit.
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Platelet count
Platelet count in blood will be done.
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
BDNF per platelet
The amount of BDNF per platelet will be calculated.
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Lactate
Lactate will be measured in blood.
Time frame: pre, 5 minutes post exercise, 1 hour post exercise, 3 hours post exercise.
Stroop test
Stroop test will be used as an assessment of cognitive functions. The number of read word will be assessed.
Time frame: pre, 15 minutes post exercise.
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Timeline of events will be same as in exercise protocol (blood withdrawal and Stroop test), except participants will not do any kind of exercise protocol, instead they will be seating.