Advanced Sperm Selection in ICSI: A Comparative Study of PICSI and ZyMot

Inclusion criteria:- * Age for women is between 20-39 years old. * Body mass index (BMI) between 19 kg/m and 35 kg/m * Normal Prolactin level. * No other endocrinological abnormality including thyroid disorder or diabetes. * With antimullerian hormone (AMH) at least 1.5 ng/ml, ), with normal progesterone level. * Primary or secondary infertility at least of 2 years duration. * No evidence of endometriosis. * No other medical or surgical disease. * The day of embryo freezing is the fifth day (day 5), and all patients who undergo the process of transferring frozen embryos. * The endometrium thickness is between 8-14 mm on day of transfer. * Inclusion criteria of male partner were; presence of ejaculate motile spermatozoa with total sperm number 1 million/ml at least and sperm motility 5% or more and all patient with high DNA fragmentation index above 35%. Exclusion criteria׃- * Women were older than 40 years of age or younger than 18 years old. * Women laparoscopically diagnosed to have endometriosis within the last 1 year. * Male infertility factor with immotile sperm.