The goal of this observational study is to investigate whether ankle evertor muscle fatigue impairs force perception and alters neuromuscular activation patterns during submaximal isometric contractions, and whether these effects differ between individuals with chronic ankle instability (CAI) and individuals without CAI.
Participants will first be familiarized with the research protocol for approximately 20 minutes. After a short break, the experimental protocol with data collection will begin and last between 45 and 60 minutes. Measurements will be performed on the side where the participant reports symptoms of chronic ankle instability. In cases of bilateral CAI, the leg with the lower CAIT score will be tested. Participants will be tested on an isokinetic dynamometer (Humac Norm, CSMi, USA) in a semi-recumbent position with the hip and knee flexed to ensure comfort and alignment. The trunk and tested leg will be secured with straps to minimize accessory movement. After initial maximal voluntary isometric contraction (MVIC) of ankle evertors, participants will perform force-matching tasks at 25% and 50% of MVIC. Each trial will consist of 5 seconds with visual feedback followed by 5 seconds without feedback, repeated three times per intensity. After completing these tasks, participants will undergo a fatigue protocol for the ankle evertors. After the fatigue protocol, participants will repeat the force-matching tasks at both 25% and 50% MVIC. The muscle fatigue protocol consists of consecutive isotonic contractions of ankle eversion, performed through the full range of motion at 30% of MVIC torque until visible fatigue. Fatigue will be defined as a clear inability to perform full range of eversion, i.e. the range of motion fell below 10% of the initial value despite evident effort by the participant. The test will be paced using a metronome, with a 1-second concentric and a 1-second eccentric phase to ensure consistent movement velocity.
Study Type
OBSERVATIONAL
Enrollment
50
Faculty of Health Sciences
Ljubljana, Slovenia
RECRUITINGConstant Error (CE)
Muscle force sense will be assessed by the accuracy of force reproduction during force-matching tasks. Constant Error (CE) will be calculated as the mean difference in force (Nm) between reproduction with and without visual feedback during the middle three seconds, averaged across three repetitions at each load level. Unit of measure: Newton-meters (Nm).
Time frame: Baseline (Day 1, prior to fatigue protocol) and immediately post-fatigue (Day 1, within 5 minutes after fatigue protocol).
Absolute Error (AE)
Muscle force sense will be assessed by the accuracy of force reproduction during force-matching tasks. Absolute Error (AE) will be calculated as the mean absolute difference in force (Nm) between reproduction with and without visual feedback during the middle three seconds, averaged across three repetitions at each load level. Unit of measure: Newton-meters (Nm).
Time frame: Baseline (Day 1, prior to fatigue protocol) and immediately post-fatigue (Day 1, within 5 minutes after fatigue protocol).
Variable Error (VE)
Muscle force sense will be assessed by the accuracy of force reproduction during force-matching tasks. Variable Error (VE) will be calculated as the standard deviation of errors across three repetitions at each load level. Unit of measure: Newton-meters (Nm).
Time frame: Time Frame: Baseline (Day 1, prior to fatigue protocol) and immediately post-fatigue (Day 1, within 5 minutes after fatigue protocol).
Coefficient of Variation (CV) of generated force
Ratio of the standard deviation to the mean force during the middle three seconds of sustained contraction in each trial, calculated separately for trials with and without visual feedback. Unit of Measure: Percentage (%).
Time frame: Baseline (Day 1, prior to fatigue protocol) and immediately post-fatigue (Day 1, within 5 minutes after fatigue protocol).
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Surface EMG root mean square (RMS) amplitude of ankle muscles
RMS amplitude from surface EMG signals of the peroneus longus, tibialis anterior, soleus, and gastrocnemius medialis will be obtained during the fatigue protocol, MVIC and force-matching task. Unit of Measure: millivolts (mV).
Time frame: Baseline (Day 1, prior to fatigue protocol) and immediately post-fatigue (Day 1, within 5 minutes after fatigue protocol).
Surface EMG median frequency of peroneus longus mucle
Median frequency of EMG signals will be obtained from the peroneus longus during the fatigue protocol, the maximal voluntary isometric contraction (MVIC) and force-matching tasks. Frequency values will be averaged across repetitions. Unit of Measure: Hertz (Hz).
Time frame: Baseline (Day 1, prior to fatigue protocol) and immediately post-fatigue (Day 1, within 5 minutes after fatigue protocol).
Maximal voluntary isometric contraction (MVIC)
MVIC of the ankle evertors will be measured at the beginning of the protocol to determine the target force for submaximal isometric contractions, and repeated immediately after the fatigue protocol to assess the effectiveness of fatigue, expressed in Newton-meters (Nm).
Time frame: Baseline (Day 1, prior to fatigue protocol) and immediately post-fatigue (Day 1, within 5 minutes after fatigue protocol).
Ratings of perceived exertion
Description: Ratings of perceived exertion (RPE) will be assessed at the end of each fatigue protocol using the Borg 10 category-ratio scale, with 0 is no effort and 10 is an extreme physical effort.
Time frame: Baseline (Day 1, prior to fatigue protocol) and immediately post-fatigue (Day 1, within 5 minutes after fatigue protocol).