Cytokines as Diagnostic Markers of Pulpal Inflammation and Their Impact on the Outcome of Vital Pulp Therapy

Mansoura University40 enrolled

Overview

intervention eligible patients with teeth diagnosed with reversible or irreversible pulpits will go for vital pulp therapy and the other group will be patients will go for extraction of caries free teeth for orthodontic reasons, blood samples will be collected from both groups to analyze the level of cytokines IL-8 and IL-10 and it's relation with the success of the pulp therapy

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

TREATMENT

Masking

NONE

Enrollment

Conditions

Reversible Pulpitis Irreversible Pulpitis

Interventions

Vital pulp therapyPROCEDURE

1. Full medical and dental history will be taken from each patient. 2. Clinical and radiographic examination (Cone beam computed tomography) for the offending tooth. 3. After anesthesia, Rubber dam is applied and complete caries removal will be done, deeply inside the cavity, the excavation will be done using low speed hand piece and excavator. 4. A blood sample will be taken from the bleeding pulp tissue on a cotton pellet for Molecular test. 5. After exposure, the pulp will be amputated to the level of the canal orifices (full pulpotomy) pulp vitality was confirmed by the presence of bleeding pulp tissue from all canals. 6. Putty MTA will gently place over the pulp. 7. The cavity will be sealed with initial deep layer of resin modified glass ionomer restoration (RMGI) and final superficial layer of composite restoration.

ExtractionPROCEDURE

1. Full medical and dental history will be taken from each patient. 2. Clinical and radiographic examination (Cone beam computed tomography) for the offending tooth. 3. After anesthesia, Rubber dam is applied. 4. A blood sample will be taken from the bleeding pulp tissue on a cotton pellet for Molecular test. 5. Extraction for orthodontic reasons.

Eligibility

Sex: ALLMin age: 16 YearsMax age: 45 Years

Medical Language ↔ Plain English

Inclusion Criteria: * The age ranges from 16-45 years medically free. * Tooth should be vital on cold testing. * Vital permanent premolar and molar on cold testing, with deep caries, subsequent pulp exposure and bleeding pulp tissue in all canals after complete pulpotomy. * Diagnosis is either reversible or irreversible pulpitis with/ without periapical rarefaction. * The tooth is restorable and free from advanced periodontal disease. * Soft tissues around the tooth are normal with no swelling or sinus tract. Exclusion Criteria: * Patients over 45 years of age. * Patients with clinical symptoms of periradicular inflammation or any periradicular radiographic lesions. * Negative results for cold and electrical pulp testing

Locations (1)

Faculty of Dentistry, Mansoura Univeristy

Al Mansurah, Egypt

Outcomes

Primary Outcomes

Clinical success

post operative pain on cold testing binary outcome (Yes or no)

Time frame: one day, one, four and twelve weeks.

Secondary Outcomes

Radiographic success

Cone Beam Computed Tomography CBCT radiographic Examination for any periodontal widening or any periapical radiolucency.

Time frame: Pre-operative , and after six months

level of cytokines

level of Interleukin 8 and Interleukin 10 will be measured using Enzyme- Linked Immunosorbent assay (ELISA) Kit

Time frame: through study completion, an average of 6 months

Data from ClinicalTrials.gov

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