Gingival enlargement is a common complication in orthodontic patients, often resulting from plaque accumulation and chronic inflammatory response during fixed appliance therapy. Surgical gingivectomy is frequently required to restore gingival contour, improve esthetics, and facilitate oral hygiene. Traditionally, gingivectomy is performed using a scalpel, but laser-assisted techniques have recently gained popularity due to their potential benefits, including reduced bleeding, postoperative discomfort, and faster healing. This randomized split-mouth clinical trial aims to compare the clinical and microbiological outcomes of gingivectomy performed with a diode laser versus conventional scalpel surgery in orthodontic patients presenting with gingival enlargement. Clinical parameters, microbial profiles, and patient-reported outcomes will be evaluated at baseline and at multiple postoperative intervals to assess the effectiveness and advantages of each technique.
Gingival enlargement is a frequent periodontal manifestation in patients undergoing fixed orthodontic treatment, primarily resulting from increased plaque retention and subsequent chronic inflammation. This condition can compromise esthetics, interfere with effective oral hygiene, and lead to periodontal complications if left untreated. Gingivectomy is the treatment of choice for removing excess gingival tissue and reestablishing physiological gingival contours. Conventional scalpel gingivectomy has been widely used; however, the advent of laser technology, particularly diode lasers, has provided an alternative surgical approach with potential clinical advantages, including better hemostasis, reduced intraoperative discomfort, faster wound healing, and less postoperative pain. This study is designed as a randomized split-mouth clinical trial to compare the clinical and microbiological outcomes of gingivectomy performed using a scalpel versus a diode laser in orthodontic patients with gingival enlargement. Eligible participants will be recruited from the Orthodontics Department of the Ho Chi Minh City National Hospital of Odonto-Stomatology. Each patient will undergo gingivectomy on two contralateral quadrants: one quadrant treated with conventional scalpel surgery (control side) and the other with diode laser surgery (test side). The allocation of quadrants will be randomized. Clinical parameters including Plaque Index (PlI), Gingival Index (GI), Probing Pocket Depth (PPD), Gingival Margin Position (GMP), and Bleeding on Probing (BoP) will be recorded at baseline and at multiple postoperative intervals. Subgingival plaque samples will be collected for microbiological analysis using real-time polymerase chain reaction (qPCR) to evaluate the presence and levels of periodontopathogenic bacteria. Patient-reported outcomes such as postoperative pain will also be assessed using a visual analog scale (VAS). All surgical procedures will be performed by the same calibrated operator to minimize variability. Standardized oral hygiene instructions will be provided to all participants throughout the study period. The collected data will be analyzed to determine differences in clinical healing, microbial changes, and patient comfort between the two treatment modalities. The findings are expected to provide evidence-based insights into the advantages and limitations of diode laser gingivectomy compared with the traditional scalpel technique in orthodontic patients.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
SINGLE
Enrollment
13
Conventional gingivectomy performed using a surgical scalpel. Gingival overgrowth was excised using standard surgical technique under local anesthesia.
Gingivectomy performed using a diode laser. Laser parameters were set according to manufacturer's recommendations. No sutures required.
University of Medicine and Pharmacy at Ho Chi Minh City - Faculty of Odonto-Stomatology
Ho Chi Minh City, Ho Chi Minh, Vietnam
Gingival margin position
Reduction in Gingival Overgrowth Index
Time frame: 1 month and 3 months after gingivectomy
Probing Pocket Depth (PPD) Reduction
Changes in probing pocket depth at baseline and follow-up to assess periodontal healing response.
Time frame: 1 month and 3 months after gingivectomy
Reduction in Gingival Index (GI)
Changes in gingival inflammation as assessed by the Löe and Silness Gingival Index at baseline, 1 month and 3 months after treatment.
Time frame: 1 month and 3 months after gingivectomy
Reduction in Plaque Index (PlI)
Changes in plaque accumulation as measured by the Silness and Löe Plaque Index at baseline, 1 month and 3 months post-treatment.
Time frame: 1 month and 3 months after gingivectomy
Reduction in Bleeding on Probing (BoP)
Changes in bleeding on probing percentage recorded at baseline, 1 month and 3 months after treatment to evaluate periodontal health response.
Time frame: 1 month and 3 months after gingivectomy
Microbiological changes in subgingival plaque
hanges in subgingival bacterial composition (e.g., total bacterial load, presence of periodontal pathogens) between baseline, 1 month and 3 months after treatment.
Time frame: 1 month and 3 months after gingivectomy
Postoperative pain score
Patient-reported pain intensity measured using the Visual Analog Scale (VAS) to compare postoperative discomfort between treatment groups.
Time frame: 1 day, 3 days and 7 days after gingivectomy
Post-surgical bleeding
Assessment of postoperative bleeding severity and duration based on clinical observation and patient reports, to compare healing response between scalpel and diode laser techniques.
Time frame: 1 day, 3 days and 7 days post-surgery
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