Investigation of Antibacterial Effect of Levobupivacaine on Staphylococcus Aureus

Dokuz Eylul University40 enrolled

Overview

This experimental in vitro study aimed to investigate the antibacterial effect of levobupivacaine on Staphylococcus aureus in a patient-controlled analgesia (PCA) model at concentrations commonly used for postoperative pain treatment (0.125%) and painless labour (0.0625%). The antibacterial activity was evaluated using colony count reduction and supported by Scanning Electron Microscope (SEM) imaging.

Study solutions were prepared as 100 mL each. Four groups were designed: * Group 1: 0.125% levobupivacaine + 4 mL fentanyl (200 mcg) * Group 2: 0.0625% levobupivacaine + 4 mL fentanyl (200 mcg) * Group 3: 4 mL fentanyl (200 mcg) * Group 4: 99 mL saline (control) Each group received 1 mL of Staphylococcus aureus 0.5 McF (1.5×10⁸ cfu/mL). All samples were infused through a Portex bacterial filter at 4 mL/h for 24 hours using PCA devices. Samples collected from bottles, filter inlets, and outlets were cultivated on Blood Agar. Colony counts were compared statistically using the Kruskal-Wallis and Mann-Whitney U tests (p\<0.05).

Study Type

INTERVENTIONAL

Allocation

NON_RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Staphylococcus Aureus Bacterial Contamination

Interventions

LevobupivacaineDRUG

Levobupivacaine is a local anesthetic used at concentrations of 0.125% and 0.0625% to evaluate its antibacterial effect against Staphylococcus aureus in an in vitro PCA model.

FentanylDRUG

Fentanyl is an opioid analgesic used at a concentration of 200 mcg/4 mL, either alone or in combination with levobupivacaine, to assess potential contribution to antibacterial activity.

SalineOTHER

Sterile saline solution (99 mL) used as the control group to compare antibacterial activity in the PCA infusion model.

Eligibility

Sex: ALL

Medical Language ↔ Plain English

Inclusion Criteria: * Laboratory study using Staphylococcus aureus bacterial cultures. * Samples exposed to different concentrations of levobupivacaine and fentanyl solutions. * Controlled laboratory environment (Dokuz Eylül University, Microbiology Laboratory). Exclusion Criteria: * No human participants included. * No animal models used. * Studies outside the defined experimental conditions were excluded.

Outcomes

Primary Outcomes

Reduction in Staphylococcus aureus Colony Count

Quantitative measurement of the antibacterial effect of levobupivacaine and fentanyl by counting Staphylococcus aureus colonies grown on Blood Agar after 24-hour PCA infusion at different concentrations.

Time frame: 24 hours

Secondary Outcomes

Morphological Changes of Staphylococcus aureus under SEM

Evaluation of ultrastructural changes in Staphylococcus aureus cells after exposure to levobupivacaine and fentanyl using Scanning Electron Microscope (SEM) imaging.

Time frame: 48

Data from ClinicalTrials.gov

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