Vitrification has become the gold standard for oocyte and embryo cryopreservation. Several commercial kits are available on the market, some are designed for specific developmental stages (e.g. oocytes, zygotes, cleavage-stage embryos or blastocysts) and others are suitable for several stages, therefore termed "universal". Oocytes, cleavage-stage embryos and blastocysts display different levels of resistance to cryopreservation, due to stage-specific properties. While the composition and the exposition protocol of stage specific media are optimized for specific developmental stages, "universal" media display a single composition, therefore exposition protocols should be adapted to each specific developmental stage to ensure optimal survival rates. The main objective of this study is to determine whether the shift from "oocyte specific" vitrification and warming media to "universal" media has an impact oocyte survival, embryological and clinical outcomes.
A retrospective, monocentric study comparing the clinical and embryological outcomes of 111 oocyte recipient cycles with ICSI from March 2016 to July 2020. Baseline characteristics (donor age and BMI, ovarian stimulation protocol, number of collected oocytes) of the 81 related donations were also analysed. Two generations of vitrification and warming media were used during this period: * "Oocyte-specific": RapidVit™ Oocyte and RapidWarm™ Oocyte, Vitrolife * "Universal": RapidVit™ Omni and RapidWarm™ Omni, Vitrolife Patients were divided in 3 groups according to the combination of the vitrification medium and the warming medium : "specific/specific" (S/S), "specific/universal" (S/U) and "universal/universal" (U/U)..
Study Type
OBSERVATIONAL
Enrollment
111
Different combinations of the vitrification and warming media were used for oocyte vitrification procedure in the context of oocyte donation
CHU Clermont-Ferrand
Clermont-Ferrand, Auvergne, France
Impact of vitrification and warming media on clinical pregnancy rate per fresh embryo transfer
number of clinical pregnancies (one or more gestational sacs visualized in ultrasonography) expressed per 100 embryo transfers
Time frame: UltraSound at 1 month after embryo transfer
Impact of vitrification and warming media on oocyte survival rate
the number of injected oocytes divided by the number of warmed oocytes, expressed as a percentage (%)
Time frame: two hours after warming procedure
Impact of vitrification and warming media on fertilization rate
number of 2PN zygotes divided by the number of injected oocytes, expressed as a percentage (%)
Time frame: 16-18 hours
Impact of vitrification and warming media on day-2 embryo percentage
number of cleavage-stage embryos obtained divided by the number of 2PN zygotes, expressed as a percentage (%)
Time frame: 43-45 hours
Impact of vitrification and warming media on top-quality embryo percentage
Number of top-quality cleavage-stage embryos obtained divided by the total number of cleavage stage embryos, expressed as a percentage (%).
Time frame: 43-45 hours
Impact of vitrification and warming media on blastulation rate
number of blastocysts obtained divided by the number of embryos subjected to extended culture, expressed as a percentage (%)
Time frame: 114-118 hours
Impact of vitrification and warming media on good quality blastocyst rate
number of good quality blastocysts (ICM and trophoectoderm grade A or B obtained divided by the total number of blastocysts obtained, expressed as a percentage (%)
Time frame: 114-118 hours
Impact of vitrification and warming media on implantation rate
number of gestational sacs with a cardiac activity visualized in ultrasonography divided by the number of embryo transferred, expressed as a percentage (%)
Time frame: 114-118 hours
Impact of vitrification and warming media on live birth rate per embryo transfer
number live births expressed per 100 embryo transfers
Time frame: 42 weeks
Impact of vitrification and warming media on miscarriage rate per embryo transfer
number miscarriages expressed per 100 embryo transfers
Time frame: up to 42 weeks
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