The goal of this clinical trial is to learn how delayed onset muscle soreness (DOMS) in the calf muscle (gastrocnemius) affects the muscles of the posterior chain and physical performance in healthy young adults. The main questions the study aims to answer are: Does DOMS in the calf muscle change the biomechanical properties (tone, stiffness, elasticity) of other muscles in the posterior chain? Does DOMS lower trunk endurance, muscle strength, balance, or jump performance? Participants will: Take part in a gastrocnemius exercise protocol designed to safely create DOMS Complete repeated assessments at baseline, immediately after exercise, and at 24, 48, and 72 hours Have their muscle properties measured with a handheld device Complete trunk endurance tests, muscle strength tests, balance tests, and a vertical jump test Provide a blood sample to measure creatine kinase (a marker of muscle damage) This research may help clinicians better understand how soreness in one muscle can influence the whole posterior chain and may guide safer training and recovery strategies.
This prospective repeated-measures clinical trial includes healthy adults aged 18-25 years. DOMS will be induced using a standardized exercise protocol targeting the gastrocnemius muscle. Outcome measures will be collected at five time points: baseline, immediately after the DOMS-inducing exercise, and 24, 48, and 72 hours post-exercise. Primary outcomes include muscle biomechanical properties such as muscle tone, stiffness, elasticity, relaxation, and creep, measured using a digital myotonometer. Trunk endurance is also assessed as a primary outcome because of the posterior chain's role in load transfer and stabilization. Secondary outcomes include: Muscle strength Dynamic balance performance Vertical jump height Pain pressure threshold Serum creatine kinase levels to confirm muscle damage To reduce assessment bias, each assessor is responsible for only one measurement type and is blinded to the DOMS-induction procedure. All assessments occur at the same time points for all participants. The study aims to provide new information about how localized muscle soreness affects chain-related muscle behavior and functional performance. Findings may contribute to improved injury-prevention, training, and rehabilitation strategies, especially for populations exposed to high mechanical load.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
14
Participants will complete an eccentric exercise protocol to safely induce delayed onset muscle soreness (DOMS) in the gastrocnemius muscle. The protocol includes: Exercise performed bilaterally, starting with the dominant leg Three sets of eccentric heel-lowering movements A 5-minute rest period between sets Participants place the foot on a step and lower the heel for 3 seconds using eccentric contraction The heel is lowered until no further downward motion is possible The opposite leg is used only to lift the body back to the starting position in 1 second A metronome set to 60 beats per minute is used to control movement speed Each set ends when the participant can no longer maintain the required tempo Participants are instructed to avoid physical activity and not take any pain-relieving medication during the 72-hour follow-up period. Intervention Model Description: All participants receive the same DOMS induction protocol.
Istinye University Topkapi Campus, Maltepe, Teyyareci Sami St. No:3,
Istanbul, Zeytinburnu, Turkey (Türkiye)
Muscle Biomechanical Properties (Muscle Tone)
Muscle tone (F) will be assessed using the MyotonPRO device. Measurements will be recorded in Hertz (Hz). Measurements will be taken three times for each muscle segment, and the average value will be calculated. Standardized anatomical landmarks were used for each muscle: Gastrocnemius: measured at the thickest part of the medial head, approximately 10 cm below the popliteal crease. The location was confirmed with a tape measure to account for individual differences. Hamstrings: measured with the participant in a prone position, at the midpoint between the ischial tuberosity and the fibular head. Gluteus maximus: measured at the thickest portion of the muscle identified by palpation while the participant was prone. Erector spinae: measured 2.5 cm lateral to the L5 spinous process in the prone position. Measurement points were marked on the skin to en
Time frame: Baseline, 24 hours post-DOMS, 48 hours post-DOMS, and 72 hours post-DOMS
Muscle Biomechanical Properties (Muscle Stiffness)
Muscle stiffness (S) will be assessed using the MyotonPRO device. Results will be reported in Newtons per meter (N/m). Measurements will be taken three times for each muscle segment, and the average value will be calculated. Standardized anatomical landmarks were used for each muscle: Gastrocnemius: measured at the thickest part of the medial head, approximately 10 cm below the popliteal crease. The location was confirmed with a tape measure to account for individual differences. Hamstrings: measured with the participant in a prone position, at the midpoint between the ischial tuberosity and the fibular head. Gluteus maximus: measured at the thickest portion of the muscle identified by palpation while the participant was prone. Erector spinae: measured 2.5 cm lateral to the L5 spinous process in the prone position. Measurement points were marked on the skin to en
Time frame: Baseline, 24 hours post-DOMS, 48 hours post-DOMS, and 72 hours post-DOMS
Muscle Biomechanical Properties (Muscle Elasticity)
Muscle elasticity will be characterized by the logarithmic decrement (D) measured using the MyotonPRO device. This is a unitless (relative) value representing the ability of the muscle to restore its shape after deformation. Measurements will be taken three times for each muscle segment, and the average value will be calculated. Standardized anatomical landmarks were used for each muscle: Gastrocnemius: measured at the thickest part of the medial head, approximately 10 cm below the popliteal crease. The location was confirmed with a tape measure to account for individual differences. Hamstrings: measured with the participant in a prone position, at the midpoint between the ischial tuberosity and the fibular head. Gluteus maximus: measured at the thickest portion of the muscle identified by palpation while the participant was prone. Erector spinae: measured 2.5 cm lateral to the L5 spinous process in the prone position. Measurement points were marked on the skin to en
Time frame: Baseline, 24 hours post-DOMS, 48 hours post-DOMS, and 72 hours post-DOMS
Muscle Biomechanical Properties (Muscle Relaxation Time)
Mechanical stress relaxation time (R) will be measured using the MyotonPRO to assess the time the muscle takes to return to rest following deformation. Results will be reported in milliseconds (ms). Measurements will be taken three times for each muscle segment, and the average value will be calculated. Standardized anatomical landmarks were used for each muscle: Gastrocnemius: measured at the thickest part of the medial head, approximately 10 cm below the popliteal crease. The location was confirmed with a tape measure to account for individual differences. Hamstrings: measured with the participant in a prone position, at the midpoint between the ischial tuberosity and the fibular head. Gluteus maximus: measured at the thickest portion of the muscle identified by palpation while the participant was prone. Erector spinae: measured 2.5 cm lateral to the L5 spinous process in the prone position. Measurement points were marked on the skin to en
Time frame: Baseline, 24 hours post-DOMS, 48 hours post-DOMS, and 72 hours post-DOMS
Muscle Biomechanical Properties (Muscle Creep)
Muscle creep will be assessed as the ratio of relaxation and deformation time (Creep (C)) using the MyotonPRO device. This parameter is a relative unit and indicates viscoelastic behavior of the muscle. Measurements will be taken three times for each muscle segment, and the average value will be calculated. Standardized anatomical landmarks were used for each muscle: Gastrocnemius: measured at the thickest part of the medial head, approximately 10 cm below the popliteal crease. The location was confirmed with a tape measure to account for individual differences. Hamstrings: measured with the participant in a prone position, at the midpoint between the ischial tuberosity and the fibular head. Gluteus maximus: measured at the thickest portion of the muscle identified by palpation while the participant was prone. Erector spinae: measured 2.5 cm lateral to the L5 spinous process in the prone position. Measurement points were marked on the skin to en
Time frame: Baseline, 24 hours post-DOMS, 48 hours post-DOMS, and 72 hours post-DOMS
Trunk Endurance Assessment (Sorenson Test)
Sorenson Test: Participants lay prone with their pelvis at the table edge and ankles secured. They held their upper body in a horizontal position, and the time they could maintain the position was recorded. The test ended when the participant could no longer keep the trunk horizontal.
Time frame: Baseline, 24 hours post-DOMS, 48 hours post-DOMS, and 72 hours post-DOMS
Trunk Endurance Assessment (Sit-up Test)
Sit-up Test: Participants sat with knees flexed at 90° and their upper body positioned at a 60° angle. Arms were crossed over the chest. Time was recorded until the participant's upper body dropped below the 60° angle.
Time frame: Baseline, 24 hours post-DOMS, 48 hours post-DOMS, and 72 hours post-DOMS
Trunk endurance assessment (Side Bridge Test-Right and Left)
Participants lay on their side and lifted their hips to form a straight line with the torso. The duration they could maintain this position was recorded. The test was performed on both sides.
Time frame: Baseline, 24 hours post-DOMS, 48 hours post-DOMS, and 72 hours post-DOMS
Muscle Strength
Muscle strength of the gastrocnemius, hamstrings, and gluteus maximus was measured using a Lafayette hand-held dynamometer with the "make test" method. The results measured with the dynamometer were recorded in Newtons. Participants pushed against the fixed device with maximal effort for 3-5 seconds in standardized positions: Gastrocnemius: foot pushing against the wall in a reverse sit-up position. Hamstrings: prone position with the knee at 30° flexion; force applied toward knee flexion. Gluteus maximus: prone with knee at 90° flexion; force applied during hip extension.
Time frame: Baseline, 24 hours post-DOMS, 48 hours post-DOMS, and 72 hours post-DOMS
Dynamic balance performance
Dynamic balance was assessed using the Y-Balance Test. Participants stood barefoot on the central platform and reached in the anterior, posteromedial, and posterolateral directions while keeping their hands on their hips. Each direction included four practice trials and three test trials for each leg. Reach distances were recorded in centimeters and normalized to leg length. A composite score was calculated using the three reach directions. Leg dominance was determined by asking which leg the participant would use to kick a ball.
Time frame: Baseline, 24 hours post-DOMS, 48 hours post-DOMS, and 72 hours post-DOMS
Vertical jump test
To assess participants' functional performance, a vertical jump test was administered. During the test, participants were asked to jump as high as possible with both feet in front of a platform fixed to the wall. First, the participant's normal arm length was determined by touching the highest point they could reach. Then, participants performed two trials to achieve the highest jump distance, and the best value was recorded. The difference between the jump distance and the normal arm length, calculated in centimeters, was used as a performance measure.
Time frame: Baseline, 24 hours post-DOMS, 48 hours post-DOMS, and 72 hours post-DOMS
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