The purpose of this clinical trial is to determine how certain food items affect oxidative stress, inflammation, and performance recovery from exercise induced muscle damage in a resistance trained population. The main questions The investigators aim to answer are the following: * Do Haskaps speed the recovery of oxidative stress and inflammation markers after an intense lower body workout in resistance trained adults? * Do Haskaps speed the recovery of performance measures after an intense lower body workout in resistance trained adults? * The data collected in this investigation may also be used to ask additional questions not yet identified. For example, the investigators may use the stored samples to evaluate how the blood metabolites of participants differ before and after intense exercise. These additional questions are called secondary analyses. Please note that no genetic analysis will be conducted and racial and ethnic differences among participants will not be used in any secondary analyses. Researches will compare Haskap juice to a color, flavor and carbohydrate matched placebo to see if Haskaps speed recovery in inflammation, oxidative stress and performance. * Participants will be asked to drink either Haskap juice or placebo and follow a low polyphenolic diet * Participants will perform an intense resistance workout * Participants will have their blood drawn before and after the workout * Performance will be analyzed at 24, 48 and 72 hours after the workout
The main goal of the clinical trial is to learn more about how the haskap berry impacts the recovery process form intense resistance training. Haskap berries are very high in several compounds that function as antioxidants, such as polyphenols and vitamin C, which have many health promoting effects. For example, antioxidants lower inflammation and oxidative stress, which is known to cause damage to cells, proteins, and DNA. Oxidative stress is a result of excess free radicals, which are unstable molecules that can be created, along with inflammation, during intense exercise. This combination of excessive harmful byproducts in the body can cause soreness and decrease muscle performance experienced after a workout. However, the antioxidants in foods have the ability to neutralize free radicals that are produced and may reduce the time to recover from exercise. To do this, a parallel, randomized, double blind, placebo controlled clinical trial of Haskap versus a placebo on resistance trained individuals will be completed. Participants will complete an intense workout consisting of barbell back squats and leg extension. Participants will drink either Haskap juice or placebo and follow a low polyphenolic diet for a total of 11 days. Participants will have their blood drawn before supplementation, before, immediately after and 8, 24, 48, and 72 hours after the workout to track recovery of blood markers. Performance will be analyzed via vertical jumps on force plates, maximal voluntary contraction of the quadriceps on a biodex, and speed of the squat at 24, 48, and 72 hours post workout.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
SUPPORTIVE_CARE
Masking
DOUBLE
Enrollment
30
A smoothie blend of berries and water
A smoothie with no polyphenolic content and matched in carbohydrate composition to the experimental haskap smoothie
Montana State University
Bozeman, Montana, United States
RECRUITINGForce Production
Maximal Voluntary Contraction (MVC) to assess force production of knee extensors at 60 degrees.
Time frame: 72 hours
Barbell Velocity
Barbell velocity will be measure to determine how squat velocity recovers over the course of the study.
Time frame: 72 hours
Vertical Jump
Force plates will be used to evaluate vertical jump height.
Time frame: 72 hours
Inflammation (pg/mL)
Serum interleukin (IL) IL-6, IL-10, IL-1ra, tumor necrosis factor alpha (TNF-alpha),
Time frame: 11 days
Lipid peroxidation
Plasma Malondialdehyde (MDA)
Time frame: 11 days
Creatine Kinase
Plasma
Time frame: 11 days
C-Reactive Protein
Plasma
Time frame: 11 days
Protein Carbonyls
Plasma
Time frame: 11 days
Soreness
Soreness will be evaluated objectively by participants drawing a line on a scale of 0-10 of how sore their legs are. 0 represents no soreness at all and 10 represents the most muscular soreness the participant has felt. A lower number could indicate less inflammation and muscular damage.
Time frame: 72 hours
Acute Diet
24-hour dietary recall using the Automated Self-Administered 24-hour Dietary. Assessment Tool (ASA24). Outcome is macronutrient and micronutrient composition of food entry.
Time frame: 9 days
Sleep Questionnaire
Participants report information on the previous night's sleep. What time they went to sleep, how long it took them to fall asleep, how many hours they slept, whether these were normal sleep and wake times and how restful they perceive their sleep to be on a scale of 1 to 10. 1 indicates their sleep was not restful at all and 10 indicates their sleep was as restful as possible.
Time frame: 11 days
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