Molecular Mechanisms Underlying Hip and Knee Osteoarthritis in Patients Undergoing Primary Elective Arthroplasty

Medical University of Warsaw1,000 enrolled

Overview

The aim of this observational study is to determine content of rare metals and other elements, along with the levels of selected immunological parameters, in the tissue of the hip or knee joint in the course of degenerative diseases. Determining potential disturbances in the levels or functions of these parameters may in the future contribute to improving treatment - for example, through supplementation of deficient metals or modulation at the immunological level. The main question it aims to answer is: Are selected rare metals, trace elements and other selected immunological parameters lowered in the tissue of the hip or knee joint as well as in serum in the course of osteoarthritis?

Study Type

OBSERVATIONAL

Enrollment

1,000

Conditions

Total Hip Arthroplasty (THA)Total Knee Arthroplasty (TKA)Hip Osteoarthritis Knee Osteoarthristis

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion Criteria: * 18 years old and more * patient qualified for primary TKA or primary THA * patient diagnosed with hip or knee osteoarthritis * a complete set of tissues of good quality and volume is available * does not meet the exclusion criteria. Exclusion Criteria: * lack of written consent to participate in the study or surgery * any private or professional relationship with the Investigators * genetic connective tissue diseases (e.g., Ehlers-Danlos syndrome and others) * genetic metabolic diseases (e.g., gout and others) * genetic diseases affecting the anatomy of the operated joint (e.g., dysplasia and others) * previous surgeries altering the anatomy of the examined joint (e.g., periacetabular osteotomy, unicompartmental endoprosthesis, and others) * patients undergoing revision after THA/TKA * autoimmune diseases in the patient's medical history * traumatic injury to the hip or knee joint * previous participation in the study.

Outcomes

Primary Outcomes

Enzymatic activity of selected proteolytic enzymes in periarticular tissue homogenate (e.g., metalloproteinases and selected serine/cysteine proteases).

Enzymatic activity assessed using biochemical/spectrofluorometric methods and normalized to total protein. Each enzyme will be reported separately. Data is planned to be reported as activity units per mg protein (e.g. U/mg protein).

Time frame: Perioperative/Periprocedural (during primary THA/TKA; index hospitalization)

Secondary Outcomes

Protein concentration of selected inflammatory markers in blood (e.g., TNF-α, IL 17α).

Protein concentrations measured by enzyme immunoassays (EIA/ELISA). Each protein will be reported separately as pg/mL.

Time frame: Perioperative/Periprocedural (day of surgery; index hospitalization for primary THA/TKA)

Trace element concentration in periarticular tissues (e.g., Zn, Cu, Fe).

Trace element content measured by Inductively Coupled Plasma - Mass Spectrometry (ICP-MS). Each element will be reported separately. The units for serum measurements is µg/L, and for other tissue is mg/kg dry weight.

Time frame: Perioperative/Periprocedural (day of surgery; index hospitalization for primary THA/TKA)

Relative mRNA expression of selected inflammatory and bone metabolism markers in periarticular tissue (e.g. TNF-α, RANK, RANKL, BMP-12, IL-17α, IL-17αR).

Relative mRNA expression measured by RT-qPCR and reported as fold change (e.g., 2\^-ΔΔCt). Each gene will be reported separately (no aggregation into a single score).

Time frame: Perioperative/Periprocedural (day of surgery; index hospitalization for primary THA/TKA)

Molecular Mechanisms Underlying Hip and Knee Osteoarthritis in Patients Undergoing Primary Elective Arthroplasty

Overview

Conditions

Eligibility

Locations (1)

Outcomes

Primary Outcomes

Secondary Outcomes

Central Contacts