High-Dairy Food Patterns and Gut-Brain Axis

Inclusion Criteria: * Men and postmenopausal women (≥ 2 years since last menstruation); * Aged between 40-75 years; * BMI between 25-35 kg/m2 (overweight or obese); * Low-to-moderate habitual dairy consumption (≤ 3 servings/day); * Fasting serum total cholesterol \< 8.0 mmol/L; * Fasting serum triacylglycerol \< 4.5 mmol/L; * Fasting plasma glucose \< 7.0 mmol/L; * Systolic blood pressure \< 160 mmHg and diastolic blood pressure \< 100 mmHg; * Stable body weight (weight gain or loss \< 3 kg in the past three months). Exclusion Criteria: * Left-handedness; * Milk protein allergy or lactose intolerance; * Current smoker, or smoking cessation \< 12 months; * Familial hypercholesterolemia; * Abuse of drugs; * Alcoholic intake \>3 standard drinks/day; * Use of medications, food products or dietary supplements affecting glucose, lipid, or blood pressure regulation, gut microbiota or mental or neurological function, judged by the principal investigator; * Use of antibiotics within the previous month; * Use of other biomedical investigational products within the previous month; * Participation in another clinical trial within the past month; * Severe medical conditions including type 2 diabetes, epilepsy, asthma, kidney failure, COPD, inflammatory bowel disease, autoimmune diseases, or rheumatoid arthritis; * History of cardiovascular events (e.g., heart attack, stroke) or active cardiovascular disease; * Contra-indications for MRI imaging (e.g. pacemaker, metal implants, claustrophobia); * Willing to donate blood starting from 8 weeks before the study begins, throughout the study, and for 4 weeks after its inclusion; * Difficult to venipuncture as evidenced during the screening visit.