This study aims to evaluate the clinical and microbiological effects of using injectable Platelet-Rich Fibrin (i-PRF) as an adjunct to non-surgical periodontal therapy (scaling and root planing) in patients with periodontitis. The study uses a split-mouth design where one side of the mouth receives the treatment with i-PRF and the other side receives standard treatment alone. Clinical parameters and bacterial counts will be compared at baseline, 1 month, and 3 months after intervention .
Patients with periodontitis will be recruited for this split-mouth randomized controlled trial. All participants will receive full-mouth supra and sub-gingival scaling and root planing (SRP). In the intervention side, i-PRF will be injected into the periodontal pockets, while the control side will receive SRP only. Clinical measurements including Probing Pocket Depth (PPD), Clinical Attachment Level (CAL), and Bleeding on Probing (BOP) will be recorded. Additionally, microbiological samples will be collected to assess the changes in the subgingival Porphyromonas gingivalis and Fusobacterium nucleatum . Follow-up visits are scheduled at 1 and 3 months post-intervention to assess the adjunctive benefit of i-PRF.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
DOUBLE
Enrollment
13
Ten ml of whole blood is collected from the participant into plain tubes and immediately centrifuged. The centrifugation protocol follows the low-speed concept (e.g., 700 rpm for 3 minutes). The upper orange-colored liquid (i-PRF) is then collected and injected subgingivally into the periodontal pockets using a 25-gauge needle immediately after scaling and root planing
Full-mouth non-surgical periodontal therapy including supra and sub-gingival scaling and root planing performed using ultrasonic devices and Gracey curettes to remove dental biofilm and calculus
Mustansiriyah University, College of Dentistry.
Baghdad, Iraq
Change in Probing Pocket Depth (PPD)
Probing pocket depth will be measured in millimeters from the gingival margin to the base of the periodontal pocket using a standardized periodontal probe (e.g., William's probe) at six sites per tooth.
Time frame: Baseline, 1 month, and 3 months post-intervention
Clinical Attachment Level (CAL)
Measurement of the distance from the cemento-enamel junction (CEJ) to the base of the periodontal pocket in millimeters.
Time frame: Baseline, 1 month, and 3 months post-intervention
Bleeding on Probing (BOP)
Assessment of the presence or absence of bleeding within 30 seconds after probing, recorded as a percentage of total sites.
Time frame: Baseline, 1 month, and 3 months post intervention
Plaque Index (PI)
Assessment of the amount of dental plaque on the tooth surfaces to evaluate the patient's oral hygiene during the study.
Time frame: Baseline, 1 month, and 3 months post intervention .
Microbiological Analysis (Bacterial Load)
Quantification of subgingival periodontal pathogens (P. gingivalis and F. nucleatum )using Quantitative Real-time PCR (qPCR) from subgingival plaque samples.
Time frame: Baseline, 1 month, and 3 months post intervention
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