Evaluation of Microbial Status in Gastritis Patients in Different Periodontal Conditions

Aydin Adnan Menderes University30 enrolled

Overview

The aim of this study to evaluate the microbial profile in gastritis and healthy patients in different periodontal conditions via next generation technologies.

This study aims to determine the density of bacteria associated with periodontal disease and to evaluate oral dysbiosis in gastritis patients using metagenomic analysis.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Periodontal Diseases Gastritis

Interventions

saliva collectionOTHER

collecting unstimulated saliva

Eligibility

Sex: ALLMin age: 20 YearsMax age: 55 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * no systemic diseases other than gastritis non-smoker Exclusion Criteria: * Participants who took regular medications that could affect of periodontal tissue or oral microbiome pregnancy or lactation Patients who received periodontal treatment within the last 6 months

Locations (1)

Aydın Adnan Menderes University

Aydin, Turkey (Türkiye)

Outcomes

Primary Outcomes

Salivary microbial profile

To compare the oral flora profiles of the study groups, a microbiome-based next-generation sequencing approach targeting the 16S rRNA gene was employed. In this metagenomic study, following DNA extraction, phylogenetic classification was performed using the V3-V4 hypervariable regions of the 16S rRNA gene. Using the obtained data, total bacterial diversity and density were compared through alpha and beta diversity analyses. Bacterial taxa that differed between the groups were identified using LEfSe analysis.

Time frame: 1 month

Data from ClinicalTrials.gov

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