Effects of Acute Sleep Deprivation on Human Behaviours

N/ANot Yet RecruitingNCT07393607

National Taiwan Normal University48 enrolled

Overview

This study will investigate whether acute sleep deprivation affects appetite, eating behavior, and physical activity in both regular exercisers and physically inactive individuals.

Prior to the main trials, participants will complete assessments of V̇O2max and maximal muscle strength within 7 to 14 days before the main trials. They will also record one week of daily physical activity, sleep patterns, and diet (including two weekdays and one weekend day) to ensure they meet the inclusion criteria. Participants will be healthy males aged 20 to 45, grouped according to their habitual activity levels. 1. Sedentary: fewer than 7,000 steps/day, no regular exercise in the past 6 months. 2. Regular Aerobic Exercise: Running or cycling at least 2-3 times/week for the past 6 months. 3. Regular Resistance Exercise: Engaging in resistance exercise at least 2-3 times/week for the past 6 months, with minimal aerobic training. Day 1: Physical activity is restricted to a minimum, with no exercise permitted. Participants are allowed to eat freely before 15:00; however, all food intake will be recorded to enable replication prior to the subsequent main trial. Napping is not permitted. Participants will arrive at the laboratory between 18:30 and 19:00. Subjective appetite sensations and food preferences will be assessed. Participants will have either an 8-hour sleep opportunity in the laboratory from 23:00 to 07:00 (i.e., normal sleep trial) or a 3-hour sleep opportunity from 03:00 to 07:00 (i.e., partial sleep deprivation trial). Day 2: Participants will wake at 07:00. After completing personal hygiene, they will rest for 20 minutes to ensure full alertness. At 07:30, a 20-minute resting metabolic rate assessment will be conducted, alongside the collection of heart rate variability and muscle oxygen saturation data. Subsequently, pulse wave velocity measurement, cognitive function tests, subjective questionnaires, and a computerized food preference test will be administered. After the baseline assessment, a 3-hour oral glucose tolerance test (OGTT) will be administered. Visual analogue scale assessments-including sleep, appetite, and mood-will be conducted every 30 minutes during the OGTT. Food preferences will be measured 180 minutes after the completion of the OGTT. Upon leaving the laboratory, participants will be provided with a pedometer and wearable devices and instructed to record dietary intake (using a food scale and photographic records), sleep, and glucose values on the experimental day and for the following four consecutive days.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Physical Activity Energy Intake Appetite Food Choice

Interventions

Normal sleepBEHAVIORAL

Participants will have an 8-hour sleep opportunity in the lab from 23:00 to 07:00.

Partial sleep deprivationBEHAVIORAL

Participants will have a 3-hour sleep opportunity in the lab from 03:00 to 07:00.

Eligibility

Sex: MALEMin age: 20 YearsMax age: 45 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Age between 20 and 45 years. * Body Mass Index (BMI) between 18.5 to 27 kg/m². * No sleep-related medical conditions and good sleep quality, defined as a Pittsburgh Sleep Quality Index (PSQI) score ≤ 5. * Usual bedtime between 22:00 and 01:00, wake-up time between 06:00 and 09:00, with an average sleep duration of 7 to 9 hours. Chronotype classified as "definitely not evening type" (score ≥ 42 on the Morningness-Eveningness Questionnaire), with no changes in sleep patterns over the past 3 months. * Without any metabolic or cardiovascular diseases, and not using medications that could affect metabolic responses. * No specific dietary habits, such as intermittent fasting or a ketogenic diet. * Maintaining a stable weight (no self-reported weight change greater than ±3 kg) for at least 3 months. * Resting blood pressure ≤ 130/80 mmHg. * No smoking or excessive alcohol consumption. * Not a shift worker. * No international travel across time zones in the past 3 months. Exclusion Criteria: * Special diets (e.g., intermittent fasting, ketogenic diet) * Recent injuries or contraindications to intense exercise.

Outcomes

Primary Outcomes

Energy intake

Energy and macronutrients intake will be collected using pictures and via a food scale

Time frame: 4 days in a free-living conditions.

Physical activity

Physical activity level and intensity will be collected using wearable devices

Time frame: 4 days in a free-living conditions.

Secondary Outcomes

Food preference

Computerised subjective food choice

Time frame: Baseline and at the end of the 3-hour oral glucose tolerance test (OGTT).

Appetite sensations

Subjective appetitive feelings will be measured using a 0-100 mm visual analog scale (VAS), with higher values indicating more intense feelings.

Time frame: At 0 (baseline), 30, 60, 90, 120, 150, and 180 minutes during the 3-hour oral glucose tolerance test (OGTT).

Mood sensations

Subjective mood feelings will be measured using a 0-100 mm visual analog scale (VAS), with higher values indicating more intense feelings.

Time frame: At 0 (baseline), 30, 60, 90, 120, 150, and 180 minutes during the 3-hour oral glucose tolerance test (OGTT).

Sleep sensations

Subjective sleep feelings will be measured using a 0-100 mm visual analog scale (VAS), with higher values indicating more intense feelings.

Time frame: At 0 (baseline), 30, 60, 90, 120, 150, and 180 minutes during the 3-hour oral glucose tolerance test (OGTT).

Sleep

Sleep duration will be collected via wearable device.

Data from ClinicalTrials.gov

This platform is for informational purposes only and does not constitute medical advice. Always consult a qualified healthcare professional.