Inspiratory muscle warm-up (IMW) is a specific pre-exercise breathing intervention designed to prepare the respiratory muscles before physical activity and potentially enhance exercise performance. Previous research suggests that IMW may acutely improve inspiratory muscle function; however, the duration of these effects during exercise and their responses under different exercise intensities remain unclear. Furthermore, placebo-controlled comparisons are limited in the current literature. The purpose of this study is to investigate the acute effects of inspiratory muscle warm-up on inspiratory muscle strength during aerobic and anaerobic exercise in trained athletes. The study will compare three experimental conditions: a standardized inspiratory muscle warm-up protocol, a placebo breathing warm-up, and a control condition without respiratory warm-up. In this repeated-measures interventional study, trained athletes aged 14-30 years will complete three experimental sessions in randomized order. The active intervention consists of inspiratory muscle warm-up performed at 40% of maximal inspiratory pressure (MIP). The placebo condition includes a low-resistance breathing protocol designed to mimic the intervention without providing a meaningful training stimulus. The control condition involves exercise performed without respiratory warm-up. Participants will perform treadmill exercise at intensity levels representing aerobic and anaerobic workloads, defined according to percentage of maximal heart rate. Inspiratory muscle strength (MIP) will be assessed at baseline, immediately after the warm-up protocols, and periodically during exercise to evaluate time-dependent changes. The primary objective is to determine how long the acute increase in inspiratory muscle strength is maintained during exercise and to compare responses between aerobic and anaerobic athletes across the three conditions. The findings may provide evidence-based guidance for integrating respiratory muscle warm-up strategies into sport-specific preparation routines.
Inspiratory muscle warm-up (IMW) has emerged as a non-pharmacological pre-exercise strategy aimed at enhancing respiratory muscle readiness prior to physical activity. The inspiratory muscles, particularly the diaphragm and accessory respiratory muscles, play a critical role in meeting increased ventilatory demands during exercise. Acute respiratory muscle fatigue has been associated with reduced performance through mechanisms such as altered ventilatory efficiency and activation of the respiratory muscle metaboreflex, which may redistribute blood flow away from locomotor muscles. While previous studies have reported beneficial acute effects of IMW on respiratory and performance parameters, the temporal characteristics of these effects during ongoing exercise remain insufficiently understood. Existing research has primarily focused on pre- and post-intervention comparisons, often evaluating inspiratory muscle strength immediately following IMW without assessing how long these acute improvements persist during exercise. Furthermore, the influence of exercise intensity domains and athlete specialization (aerobic vs. anaerobic disciplines) on the duration of IMW-induced effects has not been systematically investigated. Another limitation of prior studies is the limited use of placebo-controlled designs, which are important for distinguishing physiological effects from expectancy or psychological influences associated with warm-up interventions. The present study is designed as a repeated-measures interventional investigation to evaluate the acute time course of inspiratory muscle strength responses following inspiratory muscle warm-up under different exercise conditions. Each participant will complete three experimental sessions, including: (1) an active inspiratory muscle warm-up protocol performed at a standardized relative intensity, (2) a placebo breathing warm-up designed to mimic the intervention without providing a meaningful physiological load, and (3) a control condition without respiratory warm-up. The order of conditions will be counterbalanced to minimize order effects. Exercise trials will involve treadmill running performed at predefined intensity domains representing aerobic and anaerobic workloads based on individual maximal heart rate. Inspiratory muscle strength will be measured repeatedly throughout exercise to identify the time-dependent decline relative to baseline and post-warm-up levels. This design allows examination of both immediate and sustained effects of respiratory muscle preparation strategies. By comparing responses between athletes from predominantly aerobic and anaerobic sports backgrounds, the study seeks to explore whether differences in energy system utilization, muscle fiber composition, and ventilatory demands influence the effectiveness and duration of inspiratory muscle warm-up. The findings may contribute to a more precise understanding of respiratory muscle preparation and support evidence-based integration of breathing-specific warm-up strategies into athletic training and competition routines.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
QUADRUPLE
Enrollment
28
A standardized inspiratory muscle warm-up performed using a breathing resistance device set at 40% of maximal inspiratory pressure (MIP). Participants perform resisted inspiratory efforts prior to exercise to acutely activate respiratory muscles.
A sham breathing warm-up performed using the same device and breathing pattern as the active intervention but with resistance set at 15% of maximal inspiratory pressure (MIP), intended to minimize physiological loading while maintaining procedural similarity.
Hitit University Faculty of Sport Sciences, Exercise Physiology Laboratory
Çorum, Turkey (Türkiye)
Time to Decline in Maximal Inspiratory Pressure (MIP) During Exercise
Time (minutes) until maximal inspiratory pressure (MIP) decreases below baseline or post-warm-up levels during aerobic and anaerobic exercise following inspiratory muscle warm-up, placebo breathing warm-up, or control conditions.
Time frame: From exercise onset until the time of MIP decline during a single acute exercise testing session (up to 30 minutes).
Acute Change in Maximal Inspiratory Pressure After Warm-Up
Difference between baseline MIP and immediate post-warm-up MIP across experimental conditions.
Time frame: From exercise onset until MIP declines below baseline or post-warm-up values, assessed at minutes 2, 4, 6, 8, and 10, then at minutes 14, 18, 22, 26, and 30 (up to 30 minutes).
Repeated MIP Measurements During Exercise
Periodic maximal inspiratory pressure values measured at predefined intervals during treadmill exercise.
Time frame: Every 2 minutes during exercise.
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