Measurement of Pro-resolving Inflammation Biomarkers in Saliva, Gingival Fluid, and Dental Pulp in Patients With Symptomatic Pulpitis and Healthy Controls

N/ANot Yet RecruitingNCT07450235

Université de Nantes50 enrolled

Overview

This observational study aims to better understand inflammation inside the dental pulp (the inner tissue of the tooth) in patients with symptomatic pulpitis compared with healthy individuals. Researchers will measure inflammation-related biomarkers in saliva, gingival fluid, and dental pulp samples to determine whether these substances reflect the level of inflammation. The study also evaluates whether saliva and oral fluids could be used as simple, non-invasive tools to detect and monitor pulp inflammation. We hypothesize that patients with pulpitis will show different biomarker levels compared with healthy controls.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Pulpitis Healthy

Eligibility

Sex: ALLMin age: 18 Years

Medical Language ↔ Plain English

Inclusion criteria * Age ≥ 18 years, male or female. * Patient consulting for symptomatic pulpitis (confirmed clinical and radiographic diagnosis) or for wisdom tooth extraction (healthy subject). * Patient able to understand the research and having signed a written consent form. * Permanent teeth only. Exclusion criteria * Refusal to participate. * Pregnancy or breastfeeding. * Antibiotic or anti-inflammatory treatment in the week prior to sampling. * Clinically diagnosed inflammatory gingival or periodontal disease (gingivitis or periodontitis) * Chronic systemic inflammatory disease (e.g., uncontrolled diabetes, autoimmune diseases). * Patients under guardianship, conservatorship, or legal protection

Locations (1)

Outcomes

Primary Outcomes

Measurement Compare LXA4 and RvE1 levels in saliva, gingival crevicular fluid, and dental pulp in patients with dental pulpitis and healthy subjects.

Average concentration of LXA4 and RvE1 (expressed in pg/mL or ng/mL) measured by mass spectrometry in each type of biological sample, compared between the pulpitis group and the healthy group.

Time frame: Day 0 (initial visit)