The primary research objective of the project is to determine the role of eGC in microvascular reactivity in patients with coronary heart disease (CHD) before and after bypass grafting (cross-sectional study 2).The main objective of the project is to increase the excellence and interdisciplinarity of scientific work at MEFOS by connecting researchers from different scientific fields, improving conditions and resources for scientific work, and increasing international cooperation, in line with strategic objective 1: raising scientific excellence. The results of the project could provide pathophysiological insight into the development and maintenance of endothelial dysfunction in CVD, as well as identify new biomarkers for CVD.
The primary objective of this project is to determine the association between endothelial glycocalyx (eGC) and myocardial revascularization in patients undergoing coronary artery bypass grafting and clinical outcomes of treatment (observational prospective study 2). Specific objective 2.1: Measure eGC thickness in coronary artery disease patients before and after surgery. Specific objective 2.2: Determine the reactivity of the bypass graft sample in in vitro experiments. Specific objective 2.3: Quantify soluble markers of eGC, oxidative stress, and inflammation from serum and PBMC samples, as well as antioxidant capacity. Specific objective 2.4: Determine the association between the results of functional tests, eGC, and markers from specific objective 2.3 with clinical outcomes of treatment.
Study Type
OBSERVATIONAL
Enrollment
35
Osijek University Hospital
Osijek, Croatia
RECRUITINGEndothelial glycocalyx
Measurement of the thickness of endothelial glycocalyx in coronary heart disease patients before and after bypass surgery - measured by Glycocheck
Time frame: Perioperative/Periprocedural
Bypass graft sample reactivity
Determine the reactivity of the bypass graft sample in in vitro experiments - measured by isolated vessel setup
Time frame: Baseline, at one time point
Endothelial glycocalyx soluble markers
Quantification of soluble markers of endothelial glycocalyx from serum or plasma samples in the specified groups - measured by ELISA assays (syndecan-1, syndecan-4, glypican-1, MMP-9)
Time frame: Perioperative/Periprocedural
Intracellular oxidative stress production
Quantification of intracellular oxidative stress production from peripheral blood mononuclear cells (PBMC) in the specified groups - measured by flow cytometry (DCF-DA and DHE)
Time frame: Perioperative/Periprocedural
Inflammation
Quantification of inflammation from peripheral blood mononuclear cells (PBMC) in the specified groups - measured by Luminex assay (cytokine profile) and flow cytometry (macrophage frequency)
Time frame: Perioperative/Periprocedural
Antioxidant capacity
Quantification of antioxidative enzyme concentration and activation from serum samples in the specified groups - measured by ELISA assays and UV-Vis spectrophotometry (SOD, GPx, CAT)
Time frame: Perioperative/Periprocedural
Oxidative stress biomarkers
Quantification of oxidative stress biomarkers from serum samples in the specified groups - measured by ELISA assays (MPO, 8-iso prostaglandin F2a, OxLDL, AOPP)
Time frame: Perioperative/Periprocedural
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