Athletes often compete in the morning when they are biologically weaker; normally in competition heats or quarterfinals to qualify for the finals scheduled in the evening. Some athletes may even choose to perform at submaximal levels in these qualifying rounds, especially when they are expected to perform multiple times in the same day (such as weightlifting at the Olympic Games). Gross muscular performance such as power output or force production is greater in the evening than the morning (\~3-14% variation). Similarly, time-trial performance and repeated sprint performance (RSP; a good measure of performance in team sport) is \~3 and 5 % greater in the evening than the morning. The reason for this daily variation in performance is attributed to central factors (such as the body clock), as well as motivational and peripheral factors, including higher core and muscle temperatures in the evening compared to the morning. The body clock located within the anterior hypothalamus consists of a group of neurons known as suprachiasmatic nuclei, which are responsible for controlling the rhythm of core temperature. The most efficient nutritional ergogenic is caffeine. Recently caffeine has been investigated to reduce the negative influence of diurnal variations on repeated-sprint ability test (10 × 6 s cycle sprints, with 30 s of rest) at 60 min after ingestion of either 5 mg·kg-1 or placebo. Lopes-Silva et al. (2019) reported that caffeine supplementation did not prevent the reduction in performance in the morning. However, placebo effect can be 3-5% and hence the use of a No-pill condition would ensure that any placebo effect is accounted for and that the true potential effect of caffeine can be established. To the best of our knowledge, no study has yet investigated caffeine (CAFF), NoPill (NOPILL) or Placebo (PLAC) effects on cognitive and 4-km time-trial (TT) performance. As a diurnal variation in 4-km TT has been widely reported in a similar population. The aim of the present study is to investigate if ingesting caffeine on the day of the morning test, to improve performance.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
TREATMENT
Masking
DOUBLE
Enrollment
15
Tom Reilly Building (LJMU)
Liverpool, Merseyside, United Kingdom
Morning 4-km time-trial finishing time
4-km cycling time-trial (Watbike Pro, Nottingham UK) finishing time in minute and seconds. \~ 4-6 minutes.
Time frame: From enrolment to last experimental session (~4 weeks)
4-km cycling time-trial split times every 1 km.
4-km cycling time-trial (Watbike Pro, Nottingham UK) split times (in minutes and seconds) every 1 km.
Time frame: From familiarisation to the final experimental session (~4 weeks)
Heart rate every 1-km
Heart rate measured every 1-km during the 4-km time-trial, using a heart rate monitor.
Time frame: From familiarisation to the final experimental session (~4 weeks)
Rating of perceived exertion every 1-km
Rating of perceived exertion every 1-km for the 4-km time-trail. Measured on a 6 (no exertion) to 20 (maximal exertion) scale. Takes \~1 s.
Time frame: From familiarisation to the final experimental session (~4 weeks)
Perceived pacing taken at 2 and 4 km
Perceived pacing taken at 2 and 4 km of the 4-km time trial. The scale is a 10 cm visual analogue scale where -5 represents pacing to slow to complete the 2 km as fast as possible, zero = optimal pacing and +5 went to fast. Takes \~1 s to answer.
Time frame: From familiarisation to the final experimental session (~4 weeks)
Morning Trail Making Test time to completion in seconds (TMT; parts A and B)
To assess the effect of 300 mg caffeine (CAFF) vs placebo (PLAC) vs no-pill (NoPill) on morning Trail Making Test time to completion in seconds (TMT; parts A and B). In part A the circles are numbered 1-25, and the participant is instructed to draw lines to connect the numbers in ascending order. In part B, the circles include both numbers (1-13) and letters (A-L) and the participant is instructed to draw lines to connect the circles in ascending pattern but with the added task of alternating between numbers and letters (i.e., 1-A-2-B-3-C etc.). In both parts the participant is instructed to connect to the circles as fast as possible, without lifting the pencil from the paper. If an error is made, this is pointed out immediately and the participant is allowed to correct it. During the test, time to completion is measured, with a higher time indicative of the greater impairment.
Time frame: From enrolment to last experimental session (4 weeks)
Morning Rey Auditory Verbal Learning Test
Rey Auditory Verbal Learning Test: The RAVLT is a neuropsychological assessment designed to evaluate verbal memory. The test is designed as a list-learning paradigm in which the volunteer hears a list of 15 nouns and is asked to recall as many words from the list as possible. The number correct and the number that were given by the participant but not on the list (intrusions) are noted. This process is re-peated 4 more times. The process is repeated but with a second interference list (List B) is presented in the same manner, and the participant is asked to recall as many words as possible from List B and scoring recorded. . After the interference trial, the participant is immediately asked to recall the words from List A, which they heard five times previously and the number of correct words and intrusions are recorded. RAVTL total number, number of dis-tractions and retention are recorded and analysed.
Time frame: From familiarisation to the final experimental session (~4 weeks)
Morning Stroop word-colour interference test
Stroop word-colour interference test. The participants were asked to read out their responses to words or colours for 45 s as quickly as possible and to leave no errors uncorrected. This was filmed and the number of errors and total amount completed was recorded and analysed. The first sheet had text (red, blue, yellow, black and green) in black ink (naming word test, W). The second sheet had blocks of colour corresponding to the text on the first sheet (naming colour test, C). With the third sheet, the participants had to read out the word (which was coloured differently to the word, e.g., the word was yellow and the colour red, referred to as the naming colour of word test, CW) and for the fourth sheet, the participants had to read out the colon (which was wrongly named, e.g., the colour was yellow but the word was red, referred to as the naming of word not colour test, WC). In this fourth sheet, the words were printed in the reverse order to the third sheet.
Time frame: From familiarisation to the final experimental session (~4 weeks)
Rectal temperature @rest and after warm-up
Rectal temperature by a soft flexible rectal probe (mini-thermistor, Grant Instruments, Shepreth, UK) approximately 10 cm beyond the external anal sphincter. Duration in each session no longer than 35 mins.
Time frame: From enrolment to last experimental session (4 weeks)
Blood lactate @ rest, pre TT and after
Blood lactate (finger tip) values will betaken @ rest, pre TT and after
Time frame: From enrolment to last experimental session (4 weeks)
Mean skin temperature (Tsk)
Mean Skin temperature (Tsk) was assessed simultaneously by skin thermistors (Grant Instruments, Squirrel 2010 series, Shepreth, UK), which were placed at four locations on the left side of the body (chest \[ch\], forearm \[f\], thigh \[th\] and calf \[ca\]). Duration in each session no longer than 35 mins. Mean Tsk was calculated as follows: Tsk = (0.34 x Tch) + (0.33 x Tth) + (0.18 x Tca) + (0.15 x Tf)
Time frame: From familiarisation to the final experimental session (~4 weeks)
The mean body temperature (Tmb)
The mean body temperature (Tmb) was calculated using skin thermistors from 4 skin sites on the left side of the body (chest \[ch\], forearm \[f\], thigh \[th\] and calf \[ca\]). : Tmb = (0.64 x Tr) + (0.36 x Tsk)
Time frame: From familiarisation to the final experimental session (~4 weeks).
Morning profile of mood states questionnaire
Morning profile of mood states questionnaire (POMS; Version 32) for moods: vigour, tension, calmness, happiness, confusion, depression and fatigue on a visual analogue scale (0=not at all and 4 = extremely). Each mood is the addition of scores for the 4 questions associated with the mood. \~ 60 s each task.
Time frame: From familiarisation to the final experimental session (~4 weeks)
Sleep questions from the Liverpool Jet lag Questionnaire
Sleep questions from the Liverpool Jet lag Questionnaire: get to sleep, how well slept, waking time and alertness after waking. Measured on a -5 (later or worst) to +5 scale (more or earlier) where ZERO is compared to normal.
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Time frame: From familiarisation to the final experimental session (~4 weeks)
7-day food diary
7-day food diary that was analyzed using Nutritics® analysis software by a SENr registered Sports and Exercise Nutritionist. Data is presented in macro-nutrients and micro-nutrient diet (mg or g).
Time frame: Baseline
Habitual caffeine consumption
Habitual caffeine consumption was assessed using the caffeine consumption questionnaire (CCQ), where caffeine intake was assessed in the morning, afternoon, evening and night. Total daily intake was expressed in mg.
Time frame: Baseline
Self-reported 2-week training diary
No preference to training regarding time-of-day by a weekly self-reported 2-week training diary for type of exercise, duration, intensity and time of day. With time of day of exercise preference recorded.
Time frame: Baseline
Chronotype
Extreme chronotype (assessed via the Composite Morningness Questionnaire) to assess morning, intermediate and evening types. Where Evening type scores 22 and less, Intermediate type 22-43 and Morning type 44 and above.
Time frame: Baseline
Poor sleep quality
Poor sleep quality (as indicated by the Pittsburgh sleep quality index global score \>5).
Time frame: At the start of the study as part of exclusion criteria
Depressed mood
Depressed mood severity from the Beck depression inventory (BDI).21 questions were answered (with 4 answers, 0= I do not feel and 3 = I am so..). The values summated to scores where 0-9 is minimal depression, 10-18 mild and 19-29 moderate depression. 30-63 sever depression. This takes 60 s to complete.
Time frame: Baseline
Caffeine withdrawal symptoms score
Caffeine withdrawal symptoms (total score) assesses caffeine withdrawal symptoms. Participants were asked to rate each of the 32 questions in terms of how they were feeling at that moment on a 5-pt. scale from 0 (not at all) to 4 (extremely). Eight of the items are positively worded (e.g., energetic) and were reversed scored prior to analyses. At start and during the protocol.\]
Time frame: From familiarisation to the final experimental session (~4 weeks).