The goal of this clinical trial is to learn how different running environments affect physiological responses, emotional regulation, exercise motivation, and attentional control in healthy college students. The main questions it aims to answer are: How do different running environments (indoor treadmill, outdoor road, and outdoor grass) affect physiological stress and recovery, such as heart rate variability, blood pressure, cortisol, and blood lactate levels? Are there differences in subjective emotional experiences, perceived recovery, and exercise motivation when running in natural versus artificial environments? Does the running environment influence post-exercise cognitive performance, specifically inhibitory control and sustained attention? Researchers will compare indoor treadmill running, outdoor road running, and outdoor grass running to see if natural environments provide greater benefits for stress relief, mood improvement, and cognitive enhancement. Participants will: Attend three separate 70-minute experimental sessions (one for each environment) with at least 48 hours between sessions. Complete baseline physiological measurements (HRV, blood pressure, saliva, and blood samples), psychological questionnaires, and computerized cognitive tasks. Complete a 30-minute moderate-intensity running session in the randomly assigned environment while wearing a heart rate monitor. Repeat the physiological measurements, psychological questionnaires, and cognitive tasks immediately and 10 minutes after the running session.
This study employs a randomized, counterbalanced crossover design to investigate the acute effects of three different running environments (indoor treadmill, outdoor road, and outdoor grass) on physiological, psychological, and cognitive outcomes. Each of the 30 healthy college student participants will complete three experimental sessions corresponding to the three environments. To eliminate carry-over effects and fatigue accumulation, a minimum washout period of 48 hours is strictly mandated between each experimental session. The experimental procedures for each session are highly standardized, lasting approximately 70 minutes in total, and are structured into three main phases: Pre-Exercise Baseline Assessment (approx. 20 minutes) Upon arrival, participants will undergo a brief health and safety screening. Researchers will then collect resting baseline physiological data, including Heart Rate Variability (HRV), Blood Pressure (BP), salivary cortisol, and blood lactate samples. Following physiological sampling, participants will complete subjective psychological scales (assessing mood state, exercise enjoyment, and perceived recovery) and perform three computerized cognitive tasks: the Stroop task (assessing attention control and inhibition), the Go/No-Go task (assessing response inhibition), and the Psychomotor Vigilance Task (PVT) (assessing sustained attention and reaction speed). Running Intervention Phase (30 minutes) Participants will perform a 30-minute moderate-intensity running session in their randomly assigned environment for that session (indoor sports venue for treadmill, campus athletic track for outdoor road, or campus lawn area for grass). Exercise intensity is continuously monitored and controlled using heart rate devices by the research team to ensure safety and standardization across all conditions. Post-Exercise Assessment Phase (approx. 20 minutes) Immediately following the 30-minute run, and again at 10 minutes post-exercise, physiological measurements (HRV, BP, salivary cortisol, and blood lactate) will be repeated to evaluate the recovery process. Participants will also complete post-exercise psychological scales and retake the three computerized cognitive tasks (Stroop, Go/No-Go, PVT). This rigorous methodology ensures that any observed differences in physiological stress regulation, emotional state, and executive function can be reliably attributed to the modulatory effects of the specific running environment.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
SINGLE
Enrollment
30
20 minutes of moderate-intensity running on an indoor treadmill. Exercise intensity is monitored and controlled using heart rate devices to maintain a consistent moderate level.
20 minutes of moderate-intensity running on an outdoor athletic track (paved surface).
20 minutes of moderate-intensity running on a natural grass surface.
Shanghai University of Sport
Shanghai, Shanghai Municipality, China
Change in Salivary Cortisol Concentration
Salivary cortisol concentration will be measured to evaluate the acute endocrine and physiological stress response to different running environments.
Time frame: Baseline (pre-exercise) and 20 minutes post-exercise.
Change in Heart Rate Variability (HRV)
Heart Rate Variability (HRV) parameters, specifically RMSSD and SDNN, will be recorded to assess the acute autonomic nervous system responses and recovery. Both parameters share the exact same unit of measure, which is milliseconds (ms).
Time frame: Baseline (pre-exercise), immediately post-exercise, 5 minutes post-exercise, 10 minutes post-exercise, and 20 minutes post-exercise.
Change in Systolic and Diastolic Blood Pressure
Blood pressure will be measured in mmHg to evaluate the cardiovascular stress response across different running environments.
Time frame: Baseline (pre-exercise), immediately post-exercise, 5 minutes post-exercise, 10 minutes post-exercise, and 20 minutes post-exercise.
Change in Blood Lactate Concentration
Blood lactate will be measured using a portable analyzer to assess metabolic exertion.
Time frame: Baseline (pre-exercise) and immediately post-exercise.
Inhibitory Control Assessed by the Stroop Task: Reaction Time
The Stroop task measures inhibitory control and cognitive interference. This measure reports the reaction time in milliseconds (ms). Lower reaction times indicate better inhibitory control.
Time frame: Baseline (pre-exercise) and 20 minutes post-exercise.
Response Inhibition Assessed by the Go/No-Go Task: Reaction Time
The Go/No-Go task is used to evaluate response inhibition and impulse control. This measure reports the reaction time in milliseconds (ms) for "Go" trials. Lower values indicate faster processing speed.
Time frame: Baseline (pre-exercise) and 20 minutes post-exercise.
Change in Heart Rate
Heart rate will be monitored and recorded in beats per minute (bpm) to assess cardiovascular exertion during the intervention and the subsequent recovery rate.
Time frame: Baseline (pre-exercise), immediately post-exercise, 5 minutes post-exercise, 10 minutes post-exercise, and 20 minutes post-exercise.
Mood State Assessed by Positive and Negative Affect Schedule (PANAS)
The Positive and Negative Affect Schedule (PANAS) consists of two subscales to measure both positive affect (10 items) and negative affect (10 items) reflecting the past week. Items are rated on a 5-point scale. For the Positive Affect subscale, the minimum possible score is 10 and the maximum possible score is 50, with higher scores indicating higher levels of positive emotions (a better outcome). For the Negative Affect subscale, the minimum possible score is 10 and the maximum possible score is 50, with higher scores indicating higher levels of negative emotions (a worse outcome).
Time frame: Baseline (pre-exercise) and 20 minutes post-exercise.
Change in Exercise Enjoyment Assessed by Physical Activity Enjoyment Scale (PACES)
The Physical Activity Enjoyment Scale (PACES) is used to evaluate the participant's subjective enjoyment of the exercise session. The scale consists of 18 items, each rated on a 7-point scale (1=completely disagree, 7=completely agree). The total score ranges from 18 to 126. Higher scores indicate a greater level of exercise enjoyment (a better outcome).
Time frame: Baseline (pre-exercise) and 20 minutes post-exercise.
Habitual Sport Motivation Assessed by Sport Motivation Scale (SMS)
The Sport Motivation Scale (SMS) is used to evaluate habitual, long-term motivation toward sports (reflecting on the past year). It consists of 28 items rated on a 7-point scale (1=does not correspond at all, 7=corresponds exactly). The total global score ranges from 28 to 196. Higher scores indicate higher levels of self-determined motivation toward sports (a better outcome).
Time frame: Baseline (pre-exercise) and 20 minutes post-exercise.
Attentional Control Assessed by Attentional Control Scale (ACS)
he Attentional Control Scale (ACS) is used to assess the participant's general trait ability to control attention and focus. The scale consists of 20 items rated on a 4-point scale (1=almost never, 4=always). The total score ranges from 20 to 80. Higher scores indicate better attentional control capabilities (a better outcome).
Time frame: Baseline (pre-exercise) and 20 minutes post-exercise.
Perceived Recovery Assessed by Restoration Outcome Scale (ROS)
The Restoration Outcome Scale (ROS) evaluates physical and psychological restoration through 6 items rated on a 5-point scale. The minimum possible total score is 6 and the maximum possible total score is 30. Higher scores indicate a higher level of perceived physical and psychological recovery (a better outcome).
Time frame: Baseline (pre-exercise) and 20 minutes post-exercise.
Inhibitory Control Assessed by the Stroop Task: Accuracy
The Stroop task measures inhibitory control and cognitive interference. This measure reports the accuracy as a percentage (%). Higher percentages indicate better cognitive performance and fewer errors.
Time frame: Baseline (pre-exercise) and 20 minutes post-exercise.
Response Inhibition Assessed by the Go/No-Go Task: Accuracy
The Go/No-Go task is used to evaluate response inhibition and impulse control. This measure reports the accuracy of responses as a percentage (%). Higher percentages indicate better impulse control and fewer false alarms.
Time frame: Baseline (pre-exercise) and 20 minutes post-exercise.
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