Characterization of JAK1 and JAK2 Activation in Gingival Tissues During Homeostasis and Periodontitis

University of Chile30 enrolled

Overview

The goal of this observational study is to examine JAK1/2 pathway activation in gingival tissues from adults with healthy gingiva and those with inflamed gingiva (periodontitis). The main question it aims to answer is: Is the JAK1/2 pathway overactivated in periodontitis compared to health? A secondary outcome will be to describe the histological areas, such as epithelium or lamina propria, where the JAK1/2 pathway will be activated including the different subpopulation of cells. The protocol include one visit where the gingival biopsies are taken. Participants with healthy gingiva and periodontitis will donate gingival tissue to study the JAK1/2 pathway. All participants will receive diagnosis and treatment of the gingival condition.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Periodontitis Periodontal Diseases Periodontal Inflammation Gingival Diseases

Eligibility

Sex: ALLMin age: 18 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Adults * Sistemically healthy Exclusion Criteria: * Active Maliganancy * History of radiation to the head-neck region * Chemotherapy or radiation within the last 5 years * History of hepatitis B/C or HIV infection * Autoimmune diseases * Diagnosis of diabetes * Pregnancy or lactation * More than 3 hospitalizations over the last 3 years * Use of systemic antibiotics over the last 3 months * Use of systemic corticosteroids or immunosuppressants over the last 3 months * Use of cytokine therapy over the last 3 months * Use of large doses of pre/probiotic supplements over the last 3 months * Smoke more than 10 cigarettes per day

Outcomes

Primary Outcomes

JAK1/2 pathway activation in gingival tissues

The activation of the JAK1/2 pathway will be evaluated by the phophorilation of JAK1/2 in the gingival tissues determined by western blot. Patients will be categorizied as 0, 1, 2. 0 = no phosphorilation 1. = mild phosphorilation 2. = intense phosphorilation

Time frame: Day 1