Sebum Metabolomics and Lipidomics for Clinical Applications

A. Healthy Participants Inclusion Criteria: * Individuals aged ≥20 years, regardless of sex or ethnicity, and assessed by the investigator to be in good health and suitable for participation. * No diagnosed skin diseases or chronic systemic diseases. * No use of medications or receipt of dermatological treatments that may affect sebum secretion or the skin microbiome within the past two weeks. Exclusion Criteria: * Known allergy to Sebutape or similar adhesive tapes, or a history of related adverse reactions. * Presence of acute or chronic skin diseases, or chronic systemic diseases. * Use of medications or receipt of dermatological treatments that may affect sebum secretion or the skin microbiome within the past two weeks. * Use of lotions, sunscreen, or other skincare products that may affect skin condition within 12 hours prior to sampling. * Any other conditions deemed unsuitable for participation by the principal investigator. B. Participants with Skin Diseases or Specific Skin Conditions Inclusion Criteria: * Individuals aged ≥20 years, regardless of sex or ethnicity, and assessed by the investigator to be suitable for participation. * Diagnosed by a physician or clinically assessed as having one of the following: * Acne * Atopic dermatitis * Seborrheic dermatitis * Psoriasis * Other common skin diseases * Specific skin conditions (e.g., postmenopausal or aging-related skin changes) Exclusion Criteria: * Known allergy to Sebutape or similar adhesive tapes, or a history of related adverse reactions. * Severe disease that may compromise skin integrity or pose a risk to participant safety. * Use of lotions, sunscreen, or other skincare products that may affect skin condition within 12 hours prior to sampling. * Any other conditions deemed unsuitable for participation by the principal investigator. C. Participants Receiving Dermatological Treatments or Using Skincare Products Inclusion Criteria: * Individuals aged ≥20 years, regardless of sex or ethnicity, and assessed by the investigator to be suitable for participation. * Participants who, based on clinical needs or daily routines, are currently using or are expected to use or receive any of the following: * Physician-prescribed topical dermatological medications * Physician-prescribed systemic therapies (oral or injectable) * Routine use of general skincare products * Physician-directed non-invasive dermatological treatments Exclusion Criteria: * Known allergy to Sebutape or similar adhesive tapes, or a history of related adverse reactions. * Severe disease that may compromise skin integrity or pose a risk to participant safety. * Use of lotions, sunscreen, or other skincare products that may affect skin condition within 12 hours prior to sampling. * Any other conditions deemed unsuitable for participation by the principal investigator. D. Healthy Participants Undergoing Short-term Antibiotic Intervention Inclusion Criteria: * Individuals aged ≥20 years, regardless of sex or ethnicity. * Assessed by the investigator to be healthy, with skin condition suitable for the intervention used in this study. Exclusion Criteria: * Known allergy to Sebutape or similar adhesive tapes, or a history of related adverse reactions. * Known allergy or adverse reactions to the study intervention drug (topical antibiotic ointment containing bacitracin, polymyxin B, and neomycin). * Presence of visible skin disease, wounds, infection, inflammation, or other abnormal skin conditions at the sampling site. * Use of lotions, sunscreen, or other skincare products that may affect skin condition within 12 hours prior to sampling. * Any other conditions deemed unsuitable for participation by the principal investigator.