The purpose of this study is to assess the digestion and absorption kinetics of beef consumed alone or as part in healthy adults. In a randomized, crossover design, 10 young, healthy adult males and females aged 18-35 years, will consume a meal consisting of 25 g protein from minced beef alone or as part of a mixed-meal during two separate experimental trials. The main study endpoint is the exogenous rate of amino acid appearance in response to the ingested beef over a 10-h period.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
10
Meal consisting of 25 g protein・kg BM-1 from minced beef protein.
Maastricht University Medical Centre+
Maastricht, Limburg, Netherlands
Digestion and absorption kinetics
The exogenous rate of amino acid appearance in response to the ingested beef (expressed as μmol/kg/min). Based on tracer plasma amino acid concentrations over time (measured with ultra performance liquid chromatography-mass spectrometry).
Time frame: Over the 10 hour timeframe following beef ingestion
Whole-body protein metabolism
Whole-body protein metabolism includes measurements of protein synthesis, breakdown, oxidation, and net balance (all expressed as μmol/kg/h). Based on amino acid concentrations in plasma and breath samples over time (measured with ultra performance liquid chromatography-mass spectrometry and gas chromatography-isotope ratio mass spectrometry).
Time frame: Over the 10 hour timeframe following beef ingestion
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