The purpose of this study is to examine the effect of sideritis consumption on physiological and performance indicators in adult subjects and compare it with the control trial.
Significance of the Study: There is currently no evidence supporting an improvement in athletic performance following the use of sideritis. Despite existing studies showing that the mechanism through which sideritis acts is almost identical to that of other supplements that have been found to enhance athletic performance, direct evidence for its effectiveness remains lacking. Methodology: Initially, the participants of the study will visit the laboratory in order to be informed about the exercise research protocol. During the first visit, the participants will perform a maximal oxygen uptake (VO₂max) test on a treadmill using the RAMP protocol. In this specific method for determining maximal oxygen uptake, the treadmill speed will increase by 1 km/h every minute. Thus, the participants will reach exhaustion gradually. The participants will visit the laboratory two more times in order to perform the exercise protocols. One hour before each session, each participant will receive a dietary supplement, which will contain either 950 mg of sideritis or a specific dosage of a neutral substance (placebo). The selection of supplements will be random, as the study will be conducted using a double-blind design. This means that neither the participants nor the researcher will know which type of supplement has been consumed prior to the exercise protocol. The exercise protocol will consist of 60 minutes of treadmill running at an intensity of 70-75% of VO₂max, which has been individually determined for each participant based on the prior VO₂max test. During these 60 minutes, a gas exchange mask will be used to determine that participants are running at the specified intensity. After the 60 minutes, participants will wear the mask again and will run at a higher intensity (90-95% VO₂max) until exhaustion (Time to Exhaustion). Before each exercise protocol, and at 15, 30, 45, and 60 minutes during exercise, as well as immediately after completion, capillary blood samples will be collected to assess lactate and glucose levels. Additionally, body composition measurements will be conducted using the BIA method. Participants' dietary intake will also be recorded for the day prior to the VO₂max test, as well as for the days preceding the exercise protocol sessions. This recording will be conducted in order to determine whether any participant has consumed sideritis through their diet prior to the protocol. Statistical Analysis A 2-way repeated measures ANOVA will be performed. The statistical program SPSS 26 will be used.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
QUADRUPLE
Enrollment
12
950 mg of Sideritis Scardica (SidTea+) extract one hour pre-exercise during exercise day
950 mg of placebo one hour pre-exercise during exercise day
Exercise Biochemistry, Physiology and Nutrition Laboratory, Department of Physical Education and Sport Science, University of Thessaly
Trikala, Thessaly, Greece
Change in Blood lactate
Blood lactate concentration will be estimated using an analyzer.
Time frame: Change from baseline to 15, 30, 45, 60 minutes during exercise and immediately post-exercise
Change in mean respiratory quotient during exercise
The mean respiratory quotient during exercise will be measured using a gas analyzer
Time frame: Mean respiratory quotient will be measured during 60 minute of exercise
Change in mean oxygen consumption during exercise
Mean oxygen consumption during exercise will be measured using a gas analyzer
Time frame: Mean oxygen consumption will be measured during 60 minute of exercise
Change in mean Heart rate during exercise
Mean heart rate during exercise will be measured using a heart rate monitor
Time frame: Mean heart rate will be measured during 60 minute of exercise
Change in time to exhaustion
Time to exhaustion will be estimated using an electronic timer
Time frame: The time to exhaustion will be measured immediately after 60 minute of exercise
Change in Glucose concentration
Glucose concentration will be estimated using an analyzer.
Time frame: Change from baseline to 15, 30, 45, 60 minutes during exercise and immediately post-exercise
Change in heart rate
Heart rate will be estimated using monitor units.
Time frame: Change from baseline to 15, 30, 45, 60 minutes during exercise and immediately post-exercise
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Change in oxygen consumption
Oxygen consumption will be estimated using gas analyzer
Time frame: Change from baseline to 15, 30, 45, 60 minutes during exercise and immediately post-exercise
Change in respiratory quotient
Respiratory quotient will be estimated using gas analyzer
Time frame: Change from baseline to 15, 30, 45, 60 minutes during exercise and immediately post-exercise
Body weight
Body weight will be estimated using beam balance scale
Time frame: Baseline
Body fat (%)
Body fat will be measured by bioelectrical impedance analysis
Time frame: Baseline
Resting Heart Rate
Resting heart rate will be measured using a heart rate sensor
Time frame: Baseline
Diastolic and systolic blood pressure
Diastolic and systolic blood pressure will be measured using a manual sphygmomanometer
Time frame: Baseline
Maximal oxygen consumption (VO2max)
VO2max will be measured using an automated open-circuit spirometer
Time frame: Baseline