Research Plan for Early Disease Screening Biomarkers in Healthy Individuals

Inclusion Criteria: 1. Age: 18 - 65 years old, gender not restricted; 2. No clear history of chronic diseases (hypertension, diabetes, cardiovascular and cerebrovascular diseases, chronic liver and kidney diseases, tumors, etc.); 3. No infection history in the past 3 months (fever, cough, sore throat, etc.); 4. No use of antibiotics, hormones, immunosuppressants, etc. within the past 1 month; 5. Voluntarily sign the informed consent form and cooperate to complete the follow-up. Exclusion Criteria: 1. Abnormal laboratory indicators (ALT/AST \> 2 times the upper limit of normal value, Cr \> upper limit of normal value, main indicators of blood routine test are abnormal and have clinical significance); 2. Pregnant or lactating women; 3. Recent (within 3 months) history of surgery, trauma or blood transfusion; 4. Mental illness or cognitive impairment that makes it impossible to cooperate with the research.