Evaluation of Fluid Biomarkers Around Implants Exhibiting Early Stage Periimplant Bone Loss

Pamukkale University59 enrolled

Overview

This study aimed to compare biological mediators known to be important in peri-implant diseases in implants with and without early stage marginal bone loss. A total of 118 implants in 59 patients were designated as test and control groups. The radiographic and periodontal data of the included implants, along with peri-implant sulcus fluid samples, were evaluated.

This study included clinical and radiographic records of patients who received implant treatment in the maxilla and/or mandible between 11/2021 and 9/2024. Patients were divided into test and control groups based on the presence/absence of EMBL after prosthetic loading. Periodontal clinical measurements were recorded and peri-implant sulcus fluid (PISF) samples were collected. Sclerostin, calprotectin, type I collagen cross-linked N-telopeptide (NTx), matrix metalloproteinase (MMP)-8, and MMP-9 levels were analyzed using ELISA. Group comparisons were performed with the statistical analyses.

Study Type

OBSERVATIONAL

Enrollment

Conditions

Peri-implant Bone Loss Peri-implant Marginal Bone Loss Marginal Bone Loss Peri-Implant Health

Interventions

Collecting of peri-implant sulcus fluidOTHER

To evaluate biomarkers associated with peri-implant bone loss, periopaper was placed in the peri-implant pocket and absorbed with sulcus fluid.

Periapical radiographyDIAGNOSTIC_TEST

Parallel cone technique was used during periapical imaging to accurately measure peri-implant bone loss.

Eligibility

Sex: ALLHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * Presence of 1 mm or more of radiographic marginal bone loss within the first 3 months after implant placement * Availability of radiographic documentation * Availability of demographic data, surgical records, and data related to the implant used * Physical condition classified as Class I and II according to the American Society of Anesthesiologists (ASA) classification * Patients with no smoking history in the last 10 years * Fixed implant-supported restorations * Screw-retained implants Exclusion Criteria: * Patients receiving and/or maintaining implant treatment at centers other than the Department of Periodontology, Pamukkale University Faculty of Dentistry * Patients with incomplete or unreachable files or radiographic documentation * Patients with a known systemic disease/condition that alters their physical condition or bone metabolism * Severe bruxism cases * Pregnant and breastfeeding patients * Patients with incomplete implant-supported prosthesis * Areas grafted during or before implant surgery * Removable implant-supported restorations * Cement-retained implants * Patients undergoing peri-implantitis treatment

Locations (1)

Pamukkale University, Faculty of Dentistry, Periodontology

Denizli, Turkey (Türkiye)

Outcomes

Primary Outcomes

Calprotectin levels in peri-implant crevicular fluid

After collecting peri-implant sulcus fluid using the paper strip method, calprotectin levels in the peri-implant sulcus region were determined using the ELISA method and recorded in ng/ml.