Hypervirulent Amoxicillin-Susceptible Klebsiella Pneumoniae CC66, France, 2016-2023

Centre Hospitalier Universitaire de Nice56 enrolled

Overview

"Klebsiella pneumoniae is considered intrinsically resistant to ampicillin because of the chromosomal blaSHV β-lactamase. However, amoxicillin-susceptible isolates have been sporadically reported. This retrospective observational study analyzes amoxicillin-susceptible K. pneumoniae isolates collected between 2016 and 2023 at a university hospital in France. Whole-genome sequencing, phylogenetic analysis, and virulence and resistance profiling were performed, and clinical data were reviewed. The study aims to characterize the genomic mechanisms underlying amoxicillin susceptibility and to assess associations with specific lineages, particularly hypervirulent clonal complex 66 (CC66), and clinical features."

Study Type

OBSERVATIONAL

Enrollment

Conditions

Sepsis Infections

Interventions

Bacterial species identification by Whole Genome sequencingBIOLOGICAL

Medical Language ↔ Plain English

Inclusion Criteria: * Patients with confirmed Klebsiella pneumoniae infection. * Isolates collected between 2016 and 2023. * Isolates with confirmed susceptibility to amoxicillin. Exclusion Criteria: * Isolates not confirmed as Klebsiella pneumoniae. * Isolates without antimicrobial susceptibility confirmation.

Outcomes

Primary Outcomes

Assessment of blaSHV gene status (presence, deletion, or disruptive mutations) by whole-genome sequencing and its association with amoxicillin susceptibility.

Whole-genome sequencing was used to determine blaSHV gene status (presence, absence, or disruptive mutations)

Time frame: At isolate collection and genomic analysis (2016-2023)