The proposed clinical research project aims to explore the functional dynamics of innate immune cells in patients. The protocol includes collecting samples from episodes of inflammatory disease, from which fluid will be extracted. The sampling procedure will be carried out non-invasively for the patient. Following collection, the fluid and cells will be separated. The cytokine profile of the fluid will be analyzed using ELISA and flow cytometry to detect inflammatory cytokines in the patient. The isolated cells from the fluid will then be analyzed by flow cytometry and differentially identified based on the physiological state of the patient, as well as to identify the presence of inflammatory markers in these immune cells.
Study Type
OBSERVATIONAL
Enrollment
200
Routine digestive surgery
CHU de Nice
Nice, Alpes-Maritimes, France
RECRUITINGImmune cells dynamic
Number of cells in each immune cell population, characterization of the composition of each population based on cellular subsets,
Time frame: At the inclusion
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