This project examines whether consuming a high-protein or high-carbohydrate beverage before exercise in the heat impacts metabolism and heat balance during exercise, with the following research questions: 1. Does a high-protein vs. high-carbohydrate pre-exercise drink impact metabolism and body temperature responses during a treadmill walking in the heat? 2. Does a high-protein or high-carbohydrate drink change oxygen use and heat production during treadmill walking in the heat?
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
PREVENTION
Masking
TRIPLE
Enrollment
16
22 oz of a beverage containing 320 kilocalories (60g protein, 6g fat, 8g carbohydrate).
22oz of a carbohydrate drink containing 320 kilocalories (75g carbohydrate, 3g protein, \<1g fat)
Metabolic Heat Production
Heat production measured during exercise at 6 watts/kg, calculated based on oxygen consumption and carbon dioxide production during exercise (indirect calorimetry)
Time frame: Measured during 60 minutes of steady state exercise in 36C, 40% humidity
Core Temperature
Internal temperature change during exercise
Time frame: Measured at rest and following 60 minutes of exercise in a warm (36C, 40% humidity) environment
Urinary NGAL (ng/mL)
Measured after exercise in high-protein and high-carbohydrate trials, NGAL (neutrophil-gelatinase associated lipocalin; a marker of acute kidney injury) will be measured in urine samples in each trial to assess kidney function.
Time frame: Following 60 minutes of exercise in a warm environment (36C, 40% relative humidity)
Urine Specific Gravity (USG)
Measured in urine samples provided before and after exercise, USG will be used as a marker of urine concentration and hydration status with each intervention.
Time frame: Measured before and after 60 minutes of exercise in a warm (36C, 40%RH) environment.
Proteinuria
Measured after exercise in high-protein and high-carbohydrate trials, total protein content will be measured in urine samples in each trial to assess kidney function.
Time frame: Following 60 minutes of exercise in a warm environment (36C, 40% relative humidity)
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