Independent and Combined Effects of Short-term Sulforaphane Supplementation and Exercise on Immunometabolism in Healthy Adults

University of British Columbia20 enrolled

Overview

The study consist of 3 interventional periods which will be completed by each consenting participant in a randomized, crossover fashion. The interventional periods will be separated by a minimum 1 week washout period. One of the interventions consists of 4 days of daily supplementation with the commercially available broccoli sprout extract capsules (BrocElite). Another intervention will consist of 4 days of daily supervised treadmill running. The exercise protocol consists of 4 minutes of high-intensity running running followed by a 3 minute recovery period, repeated for a total of 4 repetitions. The last intervention combines the above two and consists of 4 days of daily supplementation and daily supervised exercise training. Before and after each intervention, basic anthropometrics (weight, heart rate, and blood pressure) will be recorded, and a venous blood sample will be collected. Blood samples will be analyzed for metabolic and inflammatory markers. Participants will be asked to refrain from structured exercise, alcohol consumption, and broccoli consumption during each of the experimental periods. Before any testing, willing participants will provide informed consent, and fill out a few demographic, and lifestyle questionnaires. The study will be conducted at the University of British Columbia in Kelowna, BC, Canada.

Study Type

INTERVENTIONAL

Allocation

RANDOMIZED

Purpose

BASIC_SCIENCE

Masking

NONE

Enrollment

Conditions

Immunometabolism

Eligibility

Sex: ALLMin age: 19 YearsMax age: 35 YearsHealthy volunteers:

Medical Language ↔ Plain English

Inclusion Criteria: * aged between 19 and 35 years * not a current smoker * physically active based on the Canadian Physical Activity Guidelines (150 minutes of weekly physical activity) Exclusion Criteria: * a history of cardiometabolic diseases or respiratory diseases (e.g., chronic obstructive pulmonary disease, asthma, diabetes, coronary artery disease) * currently following a ketogenic diet * body mass index (BMI) over 30 kg/m2 * unable to read or communicate in English * having received a vaccination or experienced an upper respiratory tract infection in the last 4 weeks * having donated more than 0.5 L of blood within the last 4 weeks * currently pregnant * have contraindications to high intensity exercise (assessed using the CSEP Get Active Questionnaire)

Outcomes

Primary Outcomes

Change in peripheral blood mononuclear cell oxygen consumption rate

OCR will be measured using a Seahorse Analyzer before and after each 4-day intervention

Time frame: 0-4 days

Secondary Outcomes

TNF-alpha secretion from LPS-stimulated whole blood

TNF-alpha secretion from LPS-stimulated whole blood will be measured via ELISA before and after each 4-day period

Time frame: 0-4 days

Change in percentage of classical monocytes before and after the 6-week intervention

The percentage of classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention

Time frame: 0-4 days

Change in percentage of intermediate monocytes before and after the 4-day intervention

The percentage of intermediate monocytes will be measured using flow cytometry at baseline and after the 4-day intervention

Time frame: 0-4 days

Change in percentage of non-classical monocytes before and after the 4-day intervention

The percentage of non-classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention

Time frame: 0-4 days

Change in TLR-4 expression on classical monocytes before and after the 4-day intervention

TLR-4 expression on classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention.

Time frame: 0-4 days

Change in TLR-4 expression on intermediate monocytes before and after the 4-day intervention

TLR-4 expression on intermediate monocytes will be measured using flow cytometry at baseline and after the 4-day intervention.

Time frame: 0-4 days

Change in TLR-4 expression on non-classical monocytes before and after the 4-day intervention

TLR-4 expression on non-classical monocytes will be measured using flow cytometry at baseline and after the 4-day intervention

Time frame: 0-4 days

Change in white blood cell counts before and after the 4-day intervention

White blood cell counts will be measured using a hematology analyzer at baseline and after the 4-day intervention

Time frame: 0-4 days

TNF-alpha secretion from PolyIC-stimulated whole blood

TNF-alpha secretion from PolyIC-stimulated whole blood will be measured via ELISA before and after each 4-day period

Time frame: 0-4 days

Change in peripheral blood mononuclear cell extracellular acidification rate before and after the 4-day period

PBMC ECAR will be measured using a Seahorse Analyzer at baseline and after the 4-day intervention

Time frame: 0-4 days

Change in PBMC mitotracker green staining before and after the 4-day period

PBMC mitotracker green will be measured using flow cytometry before and after the 4-day intervention period

Time frame: 0-4 days

Change in PBMC mitoSOX red staining before and after the 4-day period

PBMC mitoSOX red will be measured using flow cytometry before and after the 4-day intervention period

Time frame: 0-4 days

Change in PBMC mitochondrial respiration before and after the 4-day intervention period

PBMC mitochondrial respiration will be measured using high-resolution respirometry before and after the 4-day period

Time frame: 0-4 days

Independent and Combined Effects of Short-term Sulforaphane Supplementation and Exercise on Immunometabolism in Healthy Adults

Overview

Conditions

Interventions

Eligibility

Locations (1)

Outcomes

Primary Outcomes

Secondary Outcomes

Central Contacts