Diabetes mellitus is a metabolic disease that can negatively affect wound healing and tissue repair. Albumin platelet-rich fibrin (Alb-PRF) is a blood-derived biomaterial used in periodontal and regenerative treatments. It contains growth factors and cytokines that may support tissue healing. The purpose of this study is to evaluate whether type 2 diabetes affects the biological composition of Alb-PRF. For this purpose, Alb-PRF samples obtained from individuals with type 2 diabetes will be compared with samples obtained from systemically healthy individuals. A total of 20 participants will be included in the study: 10 individuals with type 2 diabetes and 10 systemically healthy individuals. Venous blood samples will be collected from each participant, and Alb-PRF will be prepared using a standardized protocol. The prepared samples will be stored at -80°C until laboratory analysis. The levels of interleukin-6 (IL-6), epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1) in Alb-PRF samples will be measured using an enzyme-linked immunosorbent assay (ELISA). These markers are related to inflammation, tissue repair, and wound healing. The results will help determine whether type 2 diabetes changes the cytokine and growth factor profile of Alb-PRF.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
NONE
Enrollment
20
Venous blood samples will be collected from participants. Alb-PRF samples will be prepared using a standardized protocol, and IL-6, EGF, and IGF-1 levels will be measured using ELISA.
İzmir Katip Çelebi University
Izmir, Cigli, Turkey (Türkiye)
IL-6 Levels in Alb-PRF samples
Interleukin-6 (IL-6) levels will be measured in Alb-PRF samples obtained from participants using ELISA.
Time frame: Baseline (Day 1)
EGF level in Alb-PRF samples
Epidermal growth factor (EGF) levels will be measured in Alb-PRF samples obtained from participants using ELISA.
Time frame: Baseline (Day 1)
IGF-1 level in Alb-PRF samples
Insulin-like growth factor-1 (IGF-1) levels will be measured in Alb-PRF samples obtained from participants using ELISA.
Time frame: Baseline (Day 1)
Correlation between age and IL-6 concentration in Alb-PRF samples
Correlation between age (years) and IL-6 concentration (pg/mL) in Alb-PRF samples measured using ELISA.
Time frame: Baseline (Day 1)
Correlation between age and EGF concentration in Alb-PRF samples
Correlation between age (years) and EGF concentration (pg/mL) in Alb-PRF samples measured using ELISA.
Time frame: Baseline (Day 1)
Correlation between age and IGF-1 concentration in Alb-PRF samples
Correlation between age (years) and IGF-1 concentration (pg/mL) in Alb-PRF samples measured using ELISA.
Time frame: Baseline (Day 1)
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