This pilot study aims to evaluate the effects of miswak use on the oral biological environment by comparing biochemical parameters obtained from saliva and dental plaque samples collected from individuals who use miswak and controls. A total of 36 healthy volunteers will be enrolled in the study, including 18 miswak+rutine oral hygiene products users and 18 rutine oral hygiene products. Periodontal clinical parameters will be recorded, and saliva and supragingival dental plaque samples will be collected from all participants. The collected samples will be analysed for biomarkers associated with periodontal inflammation and oxidative stress. Interleukin-1 beta (IL-1β), C-reactive protein (CRP), and oxidative stress levels will be measured using biochemical analysis. The findings of this study are expected to provide preliminary evidence regarding the biological effects of miswak on oral health and contribute to the current understanding of its potential role as an adjunctive oral hygiene tool.
Study Type
OBSERVATIONAL
Enrollment
36
Participants used miswak (Salvadora persica L.) as an adjunct to toothbrushing, five times per day, for one month before periodontal treatment.
Participants used a manual toothbrush twice daily for one month before periodontal treatment.
Hatay Mustafa Kemal University
Antakya, Hatay, Turkey (Türkiye)
Hatay Mustafa Kemal Üniversitesi
Hatay, Turkey (Türkiye)
Change in Salivary Interleukin-1 Beta (IL-1β) Levels
To evaluate the effect of adjunctive miswak use on salivary interleukin-1 beta (IL-1β) levels in patients with gingivitis. Salivary IL-1β concentrations will be measured using enzyme-linked immunosorbent assay (ELISA).
Time frame: Pre-op 4 weeks and after 6 weeks
Change in Clinical Periodontal Indices
To evaluate changes in clinical periodontal parameters, including Plaque Index (PI), Gingival Index (GI), and Bleeding on Probing (BOP) following adjunctive miswak use.
Time frame: Pre-op 4 weeks and after 6 weeks
Change in Salivary C-Reactive Protein (CRP) Levels
To evaluate changes in salivary C-reactive protein (CRP) concentrations as a marker of oral inflammatory status following adjunctive miswak use.
Time frame: Pre-op 4 weeks and after 6 weeks
Change in Salivary Oxidative Stress Levels
To evaluate changes in salivary oxidative stress levels following adjunctive miswak use using appropriate biochemical assays.
Time frame: Pre-op 4 weeks and after 6 weeks
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