This study investigated whether adding structured breath-holds to standard interval training improves cardiorespiratory fitness in recreational mountaineers more than interval training alone. Twenty-four male recreational mountaineers participated in an 8-week progressive treadmill interval training program. Participants were divided into two groups: a controlled-breathing group that performed structured mid-tidal breath-holds during training, and a free-breathing group that maintained unrestricted breathing. The primary outcomes measured were resting heart rate and estimated maximal oxygen uptake, along with secondary measures including blood pressure and pulmonary function indices. The study aimed to determine if this mild voluntary hypoventilation technique provides additional physiological benefits for altitude preparation.
Recreational mountaineers often train at low altitudes without access to specialized hypoxic facilities. While intermittent hypoxic training is an established preparation strategy, the required equipment is costly and inaccessible. Voluntary hypoventilation training (VHT), which involves deliberate breath-holds during exercise, has been proposed as a low-cost alternative. However, its efficacy at producing mild desaturation levels suitable for recreational settings had not been established. This retrospective clinical trial evaluated the effects of mild VHT on cardiorespiratory fitness. Twenty-four male recreational mountaineers completed a progressive 8-week treadmill interval training program (24 sessions, 65-85% heart rate reserve) between September and November 2023. Participants were allocated to either a controlled-breathing (CB) group or a free-breathing (FB) group. The CB group performed structured mid-tidal breath-holds synchronized to an electronic metronome during approximately 25% of the main training phase, targeting a peripheral oxygen saturation (SpO₂) nadir of 92-94%. The FB group completed the identical exercise protocol with unrestricted breathing, maintaining SpO₂ at or above 97%. Physiological assessments were conducted at baseline and within 48 hours post-intervention. Measurements included resting cardiovascular parameters (heart rate and blood pressure), estimated maximal oxygen uptake (VO₂max) via a modified Bruce treadmill protocol, and comprehensive pulmonary function testing (spirometry). Continuous SpO₂ monitoring was utilized during all training sessions to ensure protocol adherence and participant safety.
Study Type
INTERVENTIONAL
Allocation
NON_RANDOMIZED
Purpose
OTHER
Masking
SINGLE
Enrollment
24
A breathing maneuver consisting of structured mid-tidal breath-holds synchronized to an electronic metronome to induce mild voluntary hypoventilation (target SpO₂ nadir 92-94%) during physical exertion.
A cardiovascular exercise protocol involving supervised treadmill sessions with progressive increases in intensity (65-85% heart rate reserve) and duration (30-45 minutes) over an 8-week period.
Sport Sciences Department, University of Sistan and Baluchestan
Zahedan, Sistan and Baluchestan, Iran
Resting Heart Rate
Resting heart rate (beats per minute) recorded using a 12-lead electrocardiograph after 10 minutes of seated rest in a quiet, temperature-controlled room. The mean value from the final 2 minutes is used for analysis.
Time frame: Baseline (within 48 hours before the first training session) and post-intervention (within 48 hours after the final 8-week training session).
Estimated Maximal Oxygen Uptake (VO2max)
Estimated VO2max (mL·kg-¹·min-¹) determined using a modified Bruce treadmill protocol until volitional exhaustion. It is calculated from total treadmill time (in minutes) using the Foster equation.
Time frame: Baseline (within 48 hours before the first training session) and post-intervention (within 48 hours after the final 8-week training session).
Systolic Blood Pressure
Systolic blood pressure (mmHg) measured in triplicate using an automated oscillometric monitor; the average of 3 measurements is retained.
Time frame: Baseline and post-intervention (within 48 hours after the final 8-week training session).
Diastolic Blood Pressure
Diastolic blood pressure (mmHg) measured in triplicate using an automated oscillometric monitor; the average of 3 measurements is retained.
Time frame: Baseline and post-intervention (within 48 hours after the final 8-week training session).
Tidal Volume (TV)
Tidal volume (L) measured via calibrated spirometry; the highest value from three technically acceptable maneuvers is retained.
Time frame: Baseline and post-intervention (within 48 hours after the final 8-week training session).
Vital Capacity (VC)
Vital capacity (L) measured via calibrated spirometry; the highest value from three technically acceptable maneuvers is retained.
Time frame: Baseline and post-intervention (within 48 hours after the final 8-week training session).
Forced Vital Capacity (FVC)
Vital capacity (L) measured via calibrated spirometry; the highest value from three technically acceptable maneuvers is retained.
Time frame: Baseline and post-intervention (within 48 hours after the final 8-week training session).
Forced Expiratory Volume in 1 Second (FEV1)
Forced expiratory volume in 1 second (L) measured via calibrated spirometry; the highest value from three technically acceptable maneuvers is retained.
Time frame: Baseline and post-intervention (within 48 hours after the final 8-week training session).
Peak Expiratory Flow (PEF)
Peak expiratory flow (L/s) measured via calibrated spirometry; the highest value from three technically acceptable maneuvers is retained.
Time frame: Baseline and post-intervention (within 48 hours after the final 8-week training session).
Forced Expiratory Flow at 25-75% of FVC (FEF25-75%)
Forced expiratory flow at 25-75% of FVC (L/s) measured via calibrated spirometry; the highest value from three technically acceptable maneuvers is retained.
Time frame: Baseline and post-intervention (within 48 hours after the final 8-week training session).
Maximum Voluntary Ventilation (MVV)
Maximum voluntary ventilation (L/min) measured via calibrated spirometry; the highest value from three technically acceptable maneuvers is retained.
Time frame: Baseline and post-intervention (within 48 hours after the final 8-week training session).
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