The goal of this clinical trial is to evaluate whether hydrogen-rich water supplementation during tapering improves recovery after functional overreaching in competitive male basketball players aged 18-35 years. The main questions it aims to answer are: * Does hydrogen-rich water improve autonomic recovery after functional overreaching? * Does hydrogen-rich water improve recovery in biochemical, performance, and subjective feeling outcomes during tapering? Researchers will compare hydrogen-rich water to placebo (water containing no molecular hydrogen) to determine whether molecular hydrogen improves recovery after accumulated fatigue has been induced by functional overreaching. Participants will: * Complete a training cycle including normal training, functional overreaching, and tapering. * Consume hydrogen-rich or placebo water daily during the one-week tapering period. * Undergo repeated testing of autonomic nervous system, blood biochemical markers, performance, and subjective soreness.
The study consists of an initial screening and entry testing phase, followed by one week of normal training, two weeks of functional overreaching, and one week of tapering. During the functional overreaching period, training load is increased to approximately 120-130% of normal training load to induce accumulated fatigue. During the tapering period, training volume is reduced to approximately 50-60% of normal training volume to support recovery and performance rebound. The molecular hydrogen is administered only during the tapering period. Participants in the experimental group consume 2.5 L per day of hydrogen-rich water. The total daily dose is divided into 0.5 L in the morning, 1.5 L around training, and 0.5 L in the evening. Participants in the placebo group consume visually matched placebo water according to the same total daily intake and timing schedule. Supplementation adherence is monitored using logs, dose counts, and attendance records. Participants are randomly assigned in a 1:1 ratio to either the experimental group or the placebo group. Randomization is performed using a block scheme and a computer random number generator. Testing is performed at multiple time points: entry laboratory testing, baseline after normal training, post-functional overreaching, and post-taper. The primary interval for evaluation is the change from post-functional overreaching to post-taper. Statistical analysis is based on a t-test or analysis of variance. Data that not follow a normal distribution are treated using a logarithmic transformation or analyzed using nonparametric tests. The significance level is set at 0.05.
Study Type
INTERVENTIONAL
Allocation
RANDOMIZED
Purpose
BASIC_SCIENCE
Masking
DOUBLE
Enrollment
30
Hydrogen-rich water with molecular hydrogen concentration \>1.0 ppm.
Tap water without molecular hydrogen.
Palacky University, Faculty of Physical Culture
Olomouc, Czechia
RECRUITINGRMSSD
The root mean square of successive differences of RR intervals (RMSSD) is used as an index of parasympathetic activity and is calculated from an ECG using heart rate variability analysis.
Time frame: One-week change between post-overreaching and post-taper.
Creatine kinase
Blood creatine kinase concentration is used as a marker of muscle damage.
Time frame: One-week change between post-overreaching and post-taper.
High-sensitivity C-reactive protein
High-sensitivity C-reactive protein concentration is used as a marker of systemic inflammatory.
Time frame: One-week change between post-overreaching and post-taper.
Cortisol
Blood cortisol concentration is used as a marker of physiological stress response.
Time frame: One-week change between post-overreaching and post-taper.
Testosterone
Blood testosterone concentration is used as a marker related to anabolic-catabolic balance.
Time frame: One-week change between post-overreaching and post-taper.
Urea
Blood urea concentration is used as a marker related to metabolic and protein-turnover stress.
Time frame: One-week change between post-overreaching and post-taper.
Leukocytes
Blood leukocyte count is used as a marker of immune-related response to training.
Time frame: One-week change between post-overreaching and post-taper.
Lymphocytes
Blood lymphocyte count is used as a marker of immune-related response to training.
Time frame: One-week change between post-overreaching and post-taper.
Neutrophils
Blood lymphocyte count is used as a marker of imflammatory response to training.
Time frame: One-week change between post-overreaching and post-taper.
Reactive strength index
Reactive strength index is used as an marker of neuromuscular function of the lower limbs and is measured using a drop jump.
Time frame: One-week change between post-overreaching and post-taper.
Hangrip test
Handgrip test is used as a marker of forearm muscle strength and is measured using a dynamometer.
Time frame: One-week change between post-overreaching and post-taper.
20-m sprint
20-m sprint test is used to assess linear speed.
Time frame: One-week change between post-overreaching and post-taper.
Repeated sprint
20 x 15 m repeated sprint test is used to asses repeated sprint ability.
Time frame: One-week change between post-overreaching and post-taper.
Agility T-test
Agility T-test is used to assess planned change-of-direction performance.
Time frame: One-week change between post-overreaching and post-taper.
Basketball jump shot accuracy test
Basketball jump shot accuracy test is used to assess basketball-specific performance.
Time frame: One-week change between post-overreaching and post-taper.
Muscle soreness
Muscle soreness is assessed subjectively using a visual analog scale, which is represented by a 100 mm line marked 0 (no pain) and 100 (worst imaginable pain).
Time frame: One-week change between post-overreaching and post-taper.
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