This study is to evaluate differences in the gut and follicle/scalp microbiome signature in AGA compared to a non-AGA population. Also, to investigate differences in inflammation and other biomarkers in AGA compared to non-AGA population at baseline and after a successful treatment
Study Type
OBSERVATIONAL
Enrollment
66
Norwoord-Hamiton scale hairloss severity
Scale from 1 (no hair loss) to 7 (severe hair loss)
Time frame: 6 months
Change in hair density
Change from baseline in hair density at the vertex, crown and occipital zones Unit: u/cm2
Time frame: 6 months
Metabolomic profiling of the stool
Change in concentration of metabolites in the stool of AGA vs healthy cohorts.
Time frame: 6 months
Gut and Scalp/Follicle Microbiome Signature Differences at Baseline
Taxonomic and functional composition of the gut and scalp/hair follicle microbiome. Units: relative abundance
Time frame: Baseline
Change in proteomic profile
Change in protein expression levels in scalp swabs samples, and differences between AGA and healthy cohorts.
Time frame: 6 months
Change in Gene Expression Profiles (Transcriptomics)
Evaluation of differentially expressed genes via spatial transcriptomics and single-cell RNA sequencing (scRNA-seq) from scalp biopsy samples comparing AGA participants to healthy controls. Units: Log2 Fold Change (or "Normalized Gene Expression Counts")
Time frame: 6 months
Metabolomic profile in blood/plasma
Change in concentration of metabolites in blood/plasma
Time frame: 6 months
Proteomic profiling of the blood
Change in proteins concentration levels in blood/plasma
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Time frame: 6 months
Change in hair diameter distribution
Change from baseline in hair shaft diameter. Unit: µm
Time frame: 6 months
Eyebrow to hairline measurement
The distance from the eyebrow to the hairline is one indicator of the recession of hairline
Time frame: 6 months
Dandruff scoring
The dandruff score ranges from 0 (no dandruff state) to 5 (very important dandruff state) for both adherent and non-adherent dandruffs
Time frame: 6 months
Change in Subject-Reported Hair Density
Evaluation of the volunteer's perceived change in hair density since the start of the study (assessed via self-assessment questionnaire). Scale : improvements range from "worsened significantly" to "improved significantly"
Time frame: 6 months
Measurement of sebum levels
Measure sebum levels on the forehead and scalp
Time frame: 6 months
Gut Microbiome in Oral Minoxidil Treatment Response
Association between gut microbiome composition (metabolomics and metagenomics from stool and blood/plasma) and clinical response to oral minoxidil treatment.
Time frame: 6 months
Change in Follicular Units Distribution
Change from baseline in percentage of single, double, triple... follicular units.
Time frame: 6 months