PyroEVs/PLIN3 Mediate Lipid Droplet-Mitochondrial Coupling in Sepsis Endothelial Injury

Zhongda Hospital180 enrolled

Overview

Objective: To investigate the correlation of PyroEVs and PLIN3 with the severity and prognosis of sepsis patients. Participants: Sepsis patients meeting the Sepsis 3.0 diagnostic criteria admitted to the Intensive Care Unit of Zhongda Hospital affiliated with Southeast University, as well as healthy volunteers. Inclusion Criteria: 1. Presence of confirmed or suspected infection; 2. Sequential Organ Failure Assessment (SOFA) score increase ≥ 2 points from baseline; 3. Signed informed consent. Exclusion Criteria: Age \< 18 or \> 80 years, pregnancy, cancer patients, and individuals with diseases potentially complicated by vascular endothelial cell injury. Primary outcome: 28-day mortality, SOFA score

Study Type

OBSERVATIONAL

Enrollment

180

Conditions

Sepsis

Interventions

No Intervention: Observational CohortOTHER

No intervention

Eligibility

Sex: ALL

Medical Language ↔ Plain English

Inclusion Criteria: * a. Presence of confirmed or suspected infection; b. Sequential Organ Failure Assessment (SOFA) score increase ≥ 2 points from baseline; c. Signed informed consent. Exclusion Criteria: * Age \< 18 or \> 80 years, pregnancy, cancer patients, and individuals with diseases potentially complicated by vascular endothelial cell injury.

Outcomes

Primary Outcomes

Concentration of pyroptic extracellular vesicles (PyroEVs) in plasma

The concentration of pyroptic extracellular vesicles (PyroEVs) in plasma will be measured using nanoparticle tracking analysis. Results will be reported as particles per milliliter (particles/mL).

Time frame: Day 1

Concentration of PLIN3 in plasma

Time frame: Day 1

28-day mortality

Time frame: 28 days

SOFA score