The purpose of this study is to analyze changes in the gut microbiota of healthy adult athletes, both men and women, before and after supplementation with a dietary fiber (agavins). Each participant will be their own control, so the design will allow us to identify whether the intervention generates changes in bacterial composition and whether these changes are related to variations in physical performance. The study will determine changes in the gut microbiota, athletic performance, and markers of metabolic stress such as lactate and creatine kinase. Volunteers will be required to take 10g of agavins in warm water for 6 weeks. The gut microbiota will be evaluated through a stool sample, while athletic performance will be measured through direct calorimetry, determining VO2max. Lactate will be determined by finger prick using Accutrend Plus equipment, while creatine kinase will be measured using a blood sample taken by staff from the biomarker measurement laboratory of the Department of Nutrition and Functional Foods.
Study Type
INTERVENTIONAL
Allocation
NA
Purpose
SUPPORTIVE_CARE
Masking
NONE
Enrollment
12
tomarán 10 g en ayudas diariamente
Centro de Desarrollo de Productos Bióticos
Yautepec, Morelos, Mexico
Changes in the relative abundance of gut microbiota bacterial taxa determined by multiplex digital PCR
The relative abundance of selected gut microbiota bacterial taxa will be determined from fresh fecal samples collected at baseline and after 6 weeks of supplementation using multiplex digital PCR and reported as percentages of the total bacterial population.
Time frame: Before and after agavin supplementation, which lasts 6 weeks
Changes in metabolic stress markers (serum creatine kinase and blood lactate concentrations)
Metabolic stress will be assessed by measuring serum creatine kinase and blood lactate concentrations at baseline and after the intervention. Creatine kinase will be quantified in serum samples, and blood lactate concentration will be determined using the Accutrend Plus analyzer. Results will be reported as concentrations and changes from baseline.
Time frame: Before and after agavin supplementation, which lasts 6 weeks
Change in maximal oxygen consumption (VO₂max; mL/kg/min)
Maximal oxygen consumption (VO₂max) will be assessed using a CardioCoach Pro metabolic analyzer during a graded exercise test on a treadmill. VO₂max will be reported in mL/kg/min at baseline and after the intervention, and changes from baseline will be calculated.
Time frame: before and after the agave treatment, which lasts 6 weeks
Change in body composition
Changes in body composition will be assessed at baseline and after the intervention. Body weight (kg), body mass index (BMI; kg/m²), body fat percentage (%), and skeletal muscle mass percentage (%) will be measured and reported. Changes from baseline will be calculated for each body composition parameter.
Time frame: before and after the intervention, which lasts six weeks
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